SSEA-4+ CD34- Cells in the Adult Human Heart Show the Molecular Characteristics of a Novel Cardiomyocyte Progenitor Population

Sandstedt, Joakim; Jonsson, Marianne; Vukusic, Kristina; Dellgren, Göran; Lindahl, anders; Jeppsson, anders; asp, Julia
December 2014
Cells Tissues Organs;Dec2014, Vol. 199 Issue 2/3, p103
Academic Journal
Stage-specific embryonic antigen (SSEA) expression is used to describe the differentiation state of an embryonic stem cell (ESC). In human ESCs, SSEA-3 and SSEA-4 are highly expressed in undifferentiated cells and downregulated upon differentiation. SSEA-4 has also been described as a marker for adult stem cells in various tissues, including human neonatal cardiac tissue. However, there is currently little data on the expression of SSEAs in human adult cardiac tissue. We obtained right and left atrial biopsies from patients undergoing cardiac surgery. These were dissociated, stained for SSEAs and other cardiac stem cell markers and analyzed by flow cytometry. Directly isolated cells expressed variable levels of SSEA-1, SSEA-3 and SSEA-4. The SSEA-1+ population was established as contaminating hematopoietic cells. The SSEA-4+ population, on the other hand, could be subdivided based on the endothelial progenitor marker CD34. The SSEA-4+ CD34- population in the right atrium had a high gene expression of both early (TBX5, NKX2.5) and late (TNNT2) cardiomyocyte markers. The SSEA-4+ CD34+ population, on the other hand, overlapped with previously described C-kit+ CD45- cardiac stem cells. Primary monolayer-cultured cells retained expression of SSEAs while the cardiomyogenic specification in the SSEA-4+ CD34- population was lost. In tissue sections, SSEA-4+ cells could be identified both within and outside the myocardium. Within the myocardium, some SSEA-4+ cells coexpressed cardiomyogenic markers. In conclusion, the results show that the adult human heart expresses SSEAs and that there is a subpopulation of SSEA-4+ CD34- cells that show features of a cardiomyocyte progenitor population. © 2014 S. Karger AG, Basel


Related Articles

  • MicroRNA-363 negatively regulates the left ventricular determining transcription factor HAND1 in human embryonic stem cell-derived cardiomyocytes. Wagh, Vilas; Pomorski, Alexander; Wilschut, Karlijn J.; Piombo, Sebastian; Bernstein, Harold S. // Stem Cell Research & Therapy;2014, Vol. 5 Issue 3, p1 

    Introduction Posttranscriptional control of mRNA by microRNA (miRNA) has been implicated in the regulation of diverse biological processes from directed differentiation of stem cells through organism development. In this study we describe a unique pathway by which miRNA regulate the specialized...

  • Stem cells: Taming stem cell heterogeneity. de Souza, Natalie // Nature Methods;Jul2012, Vol. 9 Issue 7, p645 

    The article reports on gene-expression profiling of Human embryonic stem cells (hESC) cultures to identify of cellular subsets within human progenitor cell populations.

  • SCL/TAL1-mediated Transcriptional Network Enhances Megakaryocytic Specification of Human Embryonic Stem Cells. Toscano, Miguel G; Navarro-Montero, Oscar; Ayllon, Veronica; Ramos-Mejia, Veronica; Guerrero-Carreno, Xiomara; Bueno, Clara; Romero, Tamara; Lamolda, Mar; Cobo, Marien; Martin, Francisco; Menendez, Pablo; Real, Pedro J // Molecular Therapy;Jan2015, Vol. 23 Issue 1, p158 

    Human embryonic stem cells (hESCs) are a unique in vitro model for studying human developmental biology and represent a potential source for cell replacement strategies. Platelets can be generated from cord blood progenitors and hESCs; however, the molecular mechanisms and determinants...

  • Strategies for enrichment and selection of stem cell-derived tissue precursors. Bernstein, Harold S.; Hyun, William C. // Stem Cell Research & Therapy;2012, Vol. 3 Issue 3, p1 

    Human embryonic stem cells have the capacity for self-renewal and pluripotency and thus are a primary candidate for tissue engineering and regenerative therapies. These cells also provide an opportunity to study the development of human tissues ex vivo. To date, numerous human embryonic stem...

  • hESC-derived pancreatic progenitors. Sui, Lina; Liu, Guang-Hui; Belmonte, Juan Carlos Izpisua // Cell Research;May2013, Vol. 23 Issue 5, p592 

    Successful derivation of pancreatic progenitors from human embryonic stem cells (hESCs) in vitro and further differentiation towards functional β cells in vivo may create the possibility of using hESC-derived pancreatic progenitors (PPs), instead of derived β cells, as an alternative...

  • Retinoic Acid Enhances Skeletal Myogenesis in Human Embryonic Stem Cells by Expanding the Premyogenic Progenitor Population. Ryan, Tammy; Liu, Jun; Chu, Alphonse; Wang, Lisheng; Blais, Alexandre; Skerjanc, Ilona // Stem Cell Reviews & Reports;Jun2012, Vol. 8 Issue 2, p482 

    Human embryonic stem cells (hESCs) are a potential source of material for cell therapy of muscle diseases. To date, it has proven difficult to generate skeletal muscle from hESCs in high yields and within a reasonable timeframe. Further, a hESC-derived Pax3/7-positive skeletal muscle progenitor...

  • Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies. Wei, Rui; Yang, Jin; Hou, Wenfang; Liu, Guoqiang; Gao, Meijuan; Zhang, Lin; Wang, Haining; Mao, Genhong; Gao, Hongwei; Chen, Guian; Hong, Tianpei // PLoS ONE;Aug2013, Vol. 8 Issue 8, p1 

    Human embryonic stem cells (hESCs) are pluripotent and capable of undergoing multilineage differentiation into highly specialized cells including pancreatic islet cells. Thus, they represent a novel alternative source for targeted therapies and regenerative medicine for diabetes. Significant...

  • Potential for Pancreatic Maturation of Differentiating Human Embryonic Stem Cells Is Sensitive to the Specific Pathway of Definitive Endoderm Commitment. Jaramillo, Maria; Mathew, Shibin; Task, Keith; Barner, Sierra; Banerjee, Ipsita // PLoS ONE;Apr2014, Vol. 9 Issue 4, p1 

    This study provides a detailed experimental and mathematical analysis of the impact of the initial pathway of definitive endoderm (DE) induction on later stages of pancreatic maturation. Human embryonic stem cells (hESCs) were induced to insulin-producing cells following a...

  • A HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells. Später, Daniela; Abramczuk, Monika K.; Buac, Kristina; Zangi, Lior; Stachel, Maxine W.; Clarke, Jonathan; Sahara, Makoto; Ludwig, Andreas; Chien, Kenneth R. // Nature Cell Biology;Sep2013, Vol. 15 Issue 9, p1098 

    Most of the mammalian heart is formed from mesodermal progenitors in the first and second heart fields (FHF and SHF), whereby the FHF gives rise to the left ventricle and parts of the atria and the SHF to the right ventricle, outflow tract and parts of the atria. Whereas SHF progenitors have...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics