TITLE

Capacitive sensing of microcystin variants of Microcystis aeruginosa using a gold immunoelectrode modified with antibodies, gold nanoparticles and polytyramine

AUTHOR(S)
Lebogang, Lesedi; Mattiasson, Bo; Hedström, Martin
PUB. DATE
July 2014
SOURCE
Microchimica Acta;Jul2014, Vol. 181 Issue 9/10, p1009
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We report on the application of an automated and easy-to-use device to directly measure the immunoreactions between adda-specific monoclonal antibodies and microcystins. The antibodies were immobilized on a gold electrode whose surface was modified first with polytyramine and then with gold nanoparticles. The immunoreaction leads to a change in the capacitance of the system. Under optimum conditions, the sensor is capable of performing stable regeneration-assay cycles and has a low detection limit at a concentration of 0.01 pM level of microcystin-leucine-arginine (MC-LR). The surface of the biosensor can be regenerated with pH 2.5 glycine buffer which dissociates the antibody-antigen complex. The biosensor was used to monitor the production of microcystins during batch cultivation of Microcystis aeruginosa (isolated from ponds in Botswana). Liquid chromatography coupled to MS/MS detection was used to identify three variants, viz. MC-LR (995.6 Da), DmMC-LR (981.2 Da) and MC-LA (910.5 Da). [Figure not available: see fulltext.]
ACCESSION #
96701507

 

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