TITLE

Induction of Strong Anti-HIV Cellular Immunity by a Combination of Clostridium Perfringens Expressing HIV Gag and Virus Like Particles

AUTHOR(S)
Pegu, Poonam; Helmus, Ruth; Gupta, Phalguni; Tarwater, Patrick; Caruso, Lori; Chengli Shen; Ross, Ted; Yue Chen
PUB. DATE
December 2011
SOURCE
Current HIV Research;Dec2011, Vol. 9 Issue 8, p613
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The lower gastrointestinal tract is a major mucosal site of HIV entry and initial infection. Thus, the induction of strong cellular immune responses at this mucosal site will be an important feature of an effective HIV vaccine. We have used a novel prime-boost vaccination approach to induce immune responses at mucosal sites. Orally delivered recombinant Clostridium perfringens expressing HIV-1 gag (Cp-Gag) was evaluated for induction of HIV-1 Gag specific T cell responses in a prime-boost model with intranasal inoculation of HIV-1 virus like particles (VLP). HIV-1 specific cellular immune responses in both the effector (Lamina propria) and inductive sites (Peyer's patches) of the gastrointestinal (GI) tract were significantly higher in mice immunized using Cp-Gag and VLPs in a prime-boost approach compared to mice immunized with either Cp-Gag or VLPs alone. Such cellular immune response was found to be mediated by both CD8+ and CD4+ T cells. Such a strong mucosal immune response could be very useful in developing a mucosal vaccine against HIV-1.
ACCESSION #
93551699

 

Related Articles

  • A dendrite in every pie. Wonderlich, Elizabeth R.; Barratt-Boyes, Simon M. // Virulence;11/15/2012, Vol. 3 Issue 7, p647 

    Dendritic cells (DC) are a heterogeneous population of innate immune cells that are fundamental to initiating responses against invading pathogens and regulating immune responses. Myeloid DC (mDC) act as a bridge between the innate and adaptive immune response during virus infections but their...

  • Humanized Mouse Models of HIV-1 Latency. Duyne, Rachel V.; Narayanan, Aarthi; Hall, Kylene K.; Saifuddin, Mohammed; Shultz, Leonard; Kashanchi, Fatah // Current HIV Research;Dec2011, Vol. 9 Issue 8, p595 

    The existence of long-lasting cellular reservoirs of HIV-1 is one of the major hurdles in developing effective anti-retroviral therapies. These latently infected cells and tissues efficiently evade immune responses and remain dormant until activated, upon which they can generate a productive...

  • Immune activation and collateral damage in AIDS pathogenesis. Miedema, Frank; Hazenberg, Mette D.; Tesselaar, Kiki; Baarle, Debbie Van; De Boer, Rob J.; Borghans, José A. M. // Frontiers in Immunology;Sep2013, Vol. 4, p1 

    In the past decade, evidence has accumulated that human immunodeficiency virus (HIV)- induced chronic immune activation drives progression to AIDS. Studies among different monkey species have shown that the difference between pathological and non-pathological infection is determined by the...

  • Extreme Genetic Fragility of the HIV-1 Capsid. Rihn, Suzannah J.; Wilson, Sam J.; Loman, Nick J.; Alim, Mudathir; Bakker, Saskia E.; Bhella, David; Gifford, Robert J.; Rixon, Frazer J.; Bieniasz, Paul D. // PLoS Pathogens;Jun2013, Vol. 9 Issue 6, p1 

    Genetic robustness, or fragility, is defined as the ability, or lack thereof, of a biological entity to maintain function in the face of mutations. Viruses that replicate via RNA intermediates exhibit high mutation rates, and robustness should be particularly advantageous to them. The capsid...

  • Sub-Inhibitory Concentrations of Human α-defensin Potentiate Neutralizing Antibodies against HIV-1 gp41 Pre-Hairpin Intermediates in the Presence of Serum. Demirkhanyan, Lusine; Marin, Mariana; Lu, Wuyuan; Melikyan, Gregory B. // PLoS Pathogens;Jun2013, Vol. 9 Issue 6, p1 

    Human defensins are at the forefront of the host responses to HIV and other pathogens in mucosal tissues. However, their ability to inactivate HIV in the bloodstream has been questioned due to the antagonistic effect of serum. In this study, we have examined the effect of sub-inhibitory...

  • Residual viraemia does not influence 1 year virological rebound in HIV-infected patients with HIV RNA persistently below 50 copies/mL. Gianotti, Nicola; Galli, Laura; Racca, Sara; Salpietro, Stefania; Cossarini, Francesca; Spagnuolo, Vincenzo; Barda, Beatrice; Canducci, Filippo; Clementi, Massimo; Lazzarin, Adriano; Castagna, Antonella // Journal of Antimicrobial Chemotherapy (JAC);Jan2012, Vol. 67 Issue 1, p213 

    Objectives It is currently debated whether patients with residual viraemia are at higher risk of virological failure than those attaining <1 HIV RNA copy/mL. We therefore investigated the effect of residual viraemia on virological rebound. Methods We used a prospective, non-interventional,...

  • Hyperthermia Stimulates HIV-1 Replication. Roesch, Ferdinand; Meziane, Oussama; Kula, Anna; Nisole, Sébastien; Porrot, Franc¸oise; Anderson, Ian; Mammano, Fabrizio; Fassati, Ariberto; Marcello, Alessandro; Benkirane, Monsef; Schwartz, Olivier // PLoS Pathogens;Jul2012, Vol. 8 Issue 7, Special section p1 

    HIV-infected individuals may experience fever episodes. Fever is an elevation of the body temperature accompanied by inflammation. It is usually beneficial for the host through enhancement of immunological defenses. In cultures, transient non-physiological heat shock (42-45°C) and Heat Shock...

  • Global Dynamics of a Delayed HIV-1 Infection Model with CTL Immune Response. Yunfei Li; Rui Xu; Zhe Li; Shuxue Mao // Discrete Dynamics in Nature & Society;2011, Special section p1 

    A delayed HIV-1 infection model with CTL immune response is investigated. By using suitable Lyapunov functionals, it is proved that the infection-free equilibrium is globally asymptotically stable if the basic reproduction ratio for viral infection is less than or equal to unity; if the basic...

  • Transmitted Virus Fitness and Host T Cell Responses Collectively Define Divergent Infection Outcomes in Two HIV-1 Recipients. Yue, Ling; Pfafferott, Katja J.; Baalwa, Joshua; Conrod, Karen; Dong, Catherine C.; Chui, Cecilia; Rong, Rong; Claiborne, Daniel T.; Prince, Jessica L.; Tang, Jianming; Ribeiro, Ruy M.; Cormier, Emmanuel; Hahn, Beatrice H.; Perelson, Alan S.; Shaw, George M.; Karita, Etienne; Gilmour, Jill; Goepfert, Paul; Derdeyn, Cynthia A.; Allen, Susan A. // PLoS Pathogens;Jan2015, Vol. 11 Issue 1, p1 

    Control of virus replication in HIV-1 infection is critical to delaying disease progression. While cellular immune responses are a key determinant of control, relatively little is known about the contribution of the infecting virus to this process. To gain insight into this interplay between...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics