TITLE

Heterogeneity and Glycan Masking of Cell Wall Microstructures in the Stems of Miscanthus x giganteus, and Its Parents M. sinensis and M. sacchariflorus

AUTHOR(S)
Xue, Jie; Bosch, Maurice; Knox, J. Paul
PUB. DATE
November 2013
SOURCE
PLoS ONE;Nov2013, Vol. 8 Issue 11, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Plant cell walls, being repositories of fixed carbon, are important sources of biomass and renewable energy. Miscanthus species are fast growing grasses with a high biomass yield and they have been identified as potential bioenergy crops. Miscanthus x giganteus is the sterile hybrid between M. sinensis and M. sacchariflorus, with a faster and taller growth than its parents. In this study, the occurrence of cell wall polysaccharides in stems of Miscanthus species has been determined using fluorescence imaging with sets of cell wall directed monoclonal antibodies. Heteroxylan and mixed linkage-glucan (MLG) epitopes are abundant in stem cell walls of Miscanthus species, but their distributions are different in relation to the interfascicular parenchyma and these epitopes also display different developmental dynamics. Detection of pectic homogalacturonan (HG) epitopes was often restricted to intercellular spaces of parenchyma regions and, notably, the high methyl ester LM20 HG epitope was specifically abundant in the pith parenchyma cell walls of M. x giganteus. Some cell wall probes cannot access their target glycan epitopes because of masking by other polysaccharides. In the case of Miscanthus stems, masking of xyloglucan by heteroxylan and masking of pectic galactan by heteroxylan and MLG was detected in certain cell wall regions. Knowledge of tissue level heterogeneity of polysaccharide distributions and molecular architectures in Miscanthus cell wall structures will be important for both understanding growth mechanisms and also for the development of potential strategies for the efficient deconstruction of Miscanthus biomass.
ACCESSION #
92671446

 

Related Articles

  • The Implications of Lignocellulosic Biomass Chemical Composition for the Production of Advanced Biofuels. SOREK, NADAV; YEATS, TREVOR H.; SZEMENYEI, HEIDI; YOUNGS, HEATHER; SOMERVILLE, CHRIS R. // BioScience;Mar2014, Vol. 64 Issue 3, p192 

    The majority of terrestrial biomass accumulates as plant cell walls, the main structural component of leaves, stems, roots, fruits, and seeds. The main constituents of plant cell walls are lignin and polysaccharides, which can be transformed into liquid fuel molecules through chemical...

  • New developments in the conversion of woody fibre to fuel. Ball, Jim // CFA Newsletter;Mar2014, Issue 64, p12 

    The article focuses on the use of wood materials such as wood waste, as a source of renewable energy. Topics discussed include the potential use of Miscanthus grass as a source of renewable biomass energy discussed in an article published in the journal "The Garden" in June 2013 edition, use of...

  • The Impact of Harvest Timing on Properties of Miscanthus x Giganteus Biomass as a CO2 Neutral Energy Source. Bilandžija, Nikola; Leto, Josip; Kiš, Darko; Jurišić4, Vanja; Matin, Ana; Kuže, Ivan // Collegium Antropologicum;2014, Vol. 38 Issue 1 Supp, p85 

    The European Commission, through its Directive 2009/28/EC, decided to extensively support the consumption and production of »green« energy from renewable energy sources, primarily because of diminishing supplies and constant rise in fossil fuels prices and critical necessity to mitigate...

  • Empirical Evidence of Long-Distance Dispersal in Miscanthus sinensis and Miscanthus X giganteus. Quinn, Lauren D.; Matlaga, David P.; Ryan Stewart, J.; Davis, Adam S. // Invasive Plant Science & Management;Jan-Mar2011, Vol. 4 Issue 1, p142 

    Many perennial bioenergy grasses have the potential to escape cultivation and invade natural areas. We quantify dispersal, a key component in invasion, for two bioenergy candidates:Miscanthus sinensis and M × giganteus. For each species, approximately 1 × 16 caryopses dispersed...

  • Miscanthus × Giganteus straw and pellets as sustainable fuels and raw material for activated carbon. Michel, R.; Mischler, N.; Azambre, B.; Finqueneisel, G.; Machnikowski, J.; Rutkowski, P.; Zimny, T.; Weber, J. V. // Environmental Chemistry Letters;Dec2006, Vol. 4 Issue 4, p185 

    Miscanthus × Giganteus is an excellent candidate for energy cultivation. Here we report, for the first time, the results of the pyrolysis of Miscanthus × Giganteus straw or pellets both in tubular reactor (3–6 g) and in rotary kiln (10–30 g). At 400–600°C the...

  • Cell wall polysaccharide distribution in Miscanthus lutarioriparius stem using immuno-detection. Cao, Yingping; Li, Junling; Yu, Li; Chai, Guohua; He, Guo; Hu, Ruibo; Qi, Guang; Kong, Yingzhen; Fu, Chunxiang; Zhou, Gongke // Plant Cell Reports;Apr2014, Vol. 33 Issue 4, p643 

    Key message: Cell wall polysaccharides' occurrences in two internodes of different development stages in M. lutarioriparius stem were analyzed and three major differences between them were identified by cell wall polysaccharide probes. Abstract: Deposition and modification of cell wall...

  • Recognition and Degradation of Plant Cell Wall Polysaccharides by Two Human Gut Symbionts. Martens, Eric C.; Lowe, Elisabeth C.; Chiang, Herbert; Pudlo, Nicholas A.; Meng Wu; McNulty, Nathan P.; Abbott, D. Wade; Henrissat, Bernard; Gilbert, Harry J.; Bolam, David N.; Gordon, Jeffrey I. // PLoS Biology;Dec2011, Vol. 9 Issue 12, p1 

    Symbiotic bacteria inhabiting the human gut have evolved under intense pressure to utilize complex carbohydrates, primarily plant cell wall glycans in our diets. These polysaccharides are not digested by human enzymes, but are processed to absorbable short chain fatty acids by gut bacteria. The...

  • 2-Fluoro-L-Fucose Is a Metabolically Incorporated Inhibitor of Plant Cell Wall Polysaccharide Fucosylation. Villalobos, Jose A.; Yi, Bo R.; Wallace, Ian S. // PLoS ONE;9/28/2015, Vol. 10 Issue 9, p1 

    The monosaccharide L-fucose (L-Fuc) is a common component of plant cell wall polysaccharides and other plant glycans, including the hemicellulose xyloglucan, pectic rhamnogalacturonan-I (RG-I) and rhamnogalacturonan-II (RG-II), arabinogalactan proteins, and N-linked glycans. Mutations...

  • Bioenergy beckons. Bennett, Bryony // Ecos;Jan-Mar99, Issue 98, p25 

    Examines the potential of biomass as a source of renewable energy in Australia. Cost of producing biomass energy compared to other sources; Interest of electric utilities in expanding their `green power' portfolio; Assessment of sugar processors of the potential for electricity production. ...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics