TITLE

Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production

AUTHOR(S)
Hui Shen; Poovaiah, Charleson R.; Ziebell, Angela; Tschaplinski, Timothy J.; Pattathil, Sivakumar; Gjersing, Erica; Engle, Nancy L.; Katahira, Rui; Yunqiao Pu; Sykes, Robert; Fang Chen; Ragauskas, Arthur J.; Mielenz, Jonathan R.; Hahn, Michael G.; Davis, Mark; Neal Stewart Jr., C.; Dixon, Richard A.
PUB. DATE
June 2013
SOURCE
Biotechnology for Biofuels;2013, Vol. 6 Issue 1, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Lignocellulosic biomass is one of the most promising renewable and clean energy resources to reduce greenhouse gas emissions and dependence on fossil fuels. However, the resistance to accessibility of sugars embedded in plant cell walls (so-called recalcitrance) is a major barrier to economically viable cellulosic ethanol production. A recent report from the US National Academy of Sciences indicated that, "absent technological breakthroughs", it was unlikely that the US would meet the congressionally mandated renewable fuel standard of 35 billion gallons of ethanol-equivalent biofuels plus 1 billion gallons of biodiesel by 2022. We here describe the properties of switchgrass (Panicum virgatum) biomass that has been genetically engineered to increase the cellulosic ethanol yield by more than 2-fold. Results: We have increased the cellulosic ethanol yield from switchgrass by 2.6-fold through overexpression of the transcription factor PvMYB4. This strategy reduces carbon deposition into lignin and phenolic fermentation inhibitors while maintaining the availability of potentially fermentable soluble sugars and pectic polysaccharides. Detailed biomass characterization analyses revealed that the levels and nature of phenolic acids embedded in the cell-wall, the lignin content and polymer size, lignin internal linkage levels, linkages between lignin and xylans/ pectins, and levels of wall-bound fucose are all altered in PvMYB4-OX lines. Genetically engineered PvMYB4-OX switchgrass therefore provides a novel system for further understanding cell wall recalcitrance. Conclusions: Our results have demonstrated that overexpression of PvMYB4, a general transcriptional repressor of the phenylpropanoid/lignin biosynthesis pathway, can lead to very high yield ethanol production through dramatic reduction of recalcitrance. MYB4-OX switchgrass is an excellent model system for understanding recalcitrance, and provides new germplasm for developing switchgrass cultivars as biomass feedstocks for biofuel production.
ACCESSION #
88012546

 

Related Articles

  • Fuelling a greener future. PAGE, ROSALYN // Choice;Nov2010, p18 

    This article explores the support and criticism received by the use of biofuels in Australia. It commends biofuels such as ethanol for aiming to reduce greenhouse gas emissions, improve air quality and provide alternative fuel sources. It also discusses the move of the government to review its...

  • ASSESSMENT OF GREENHOUSE GAS EMISSIONS IN ROMANIA -- CASE STUDY FOR RAPE CROP. Calciu, Irina; Vizitiu, Olga; Simota, Catalin; Mihalache, Mircea // Proceedings of the International Multidisciplinary Scientific Ge;2014, Vol. 1, p25 

    Use of renewable energy resources is regulated by EU Directive 2009/28/CE from April 23rd 2009. The reduction of the greenhouse gas emissions coming from the biofuels and bioliquids use is calculated according to the Article 19 of the Directive and is based on typical and default values. In this...

  • Biofuels Gain Energy Balance.  // C3 Views;Oct2008, Issue 25, p4 

    The article compares the efficiency of biofuels and other fossil fuels. According to reliable figures which are emerging from studies, biofuels can be significantly more efficient and result in lower greenhouse gas emissions than liquid fossil fuels. It informs about the energy required to...

  • Technoeconomic Assessment on Innovative Biofuel Technologies: The Case of Microalgae. Ribeiro, Lauro AndrĂ©; da Silva, PatrĂ­cia Pereira // ISRN Renewable Energy;2012, p1 

    The article focuses on the innovative biofuel technologies and energy sources that must replace fossil fuels and reduce emissions of greenhouse gases. It discusses the biofuel production from oils of microalgae. It states that the algal biodiesel can be produced by using carbon dioxide released...

  • Using microorganisms to brew biofuels. Rao, Reeta; Dufour, Nicholas; Swana, Jeffrey // In Vitro Cellular & Developmental Biology Plant;Dec2011, Vol. 47 Issue 6, p637 

    Interest in alternative fuel sources has grown in recent years in response to a confluence of factors, including concerns over our reliance on and increasing demand for fossil fuels as well as the deleterious environmental effects of fossil fuel extraction and utilization. The use of...

  • Comparison of Pretreatments for Lignocellulosic Biomass. Dong Yang; Shijun Wei; Qingmei Wen; Xiaojie Zhang // Advanced Materials Research;2014, Vol. 1008-1009, p111 

    Ethanol is a kind of basic biomass liquid energy, the conversion of biomass to ethanol has been studied extensively. The process of biomass pretreatment can change its physical and chemical properties, and these changes affect the chemical conversion of biomass products in quality and yield of...

  • Are there Carbon Savings from U.S. Biofuel Policies? The Critical Importance of Accounting for Leakage in Land and Fuel Markets. Bento, Antonio M.; Klotz, Richard; Landry, Joel R. // Energy Journal;Jul2015, Vol. 36 Issue 3, p75 

    We develop an analytical and numerical multi-market model that integrates land, fuel, and food markets, and link it with an emissions model to quantify the importance of carbon leakage relative to the intended emissions savings resulting from the Renewable Fuel Standard (RFS) for conventional...

  • A bioeconomy to fight climate change. van Renssen, Sonja // Nature Climate Change;Nov2014, Vol. 4 Issue 11, p951 

    The article offers information regarding the move of European Union (EU) to launch its vision for a bioeconomy, wherein biomass will replace fossil fuels from plastics to coal plants to airplanes. It discusses the use of biomass for generating energy in EU countries, helping them to meet...

  • Economic feasibility and environmental life cycle assessment of ethanol production from lignocellulosic feedstock in Pacific Northwest U.S. Juneja, Ankita; Kumar, Deepak; Murthy, Ganti S. // Journal of Renewable & Sustainable Energy;Mar2013, Vol. 5 Issue 2, p023142 

    Bioethanol produced from the lignocellulosic feedstock is a potential alternative to fossil fuels in transportation sector and can help in reducing environmental burdens. Straw produced from perennial ryegrass (PR) and wheat is a non-food, cellulosic biomass resource available in abundance in...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics