Proteomic analysis of nipple aspirate fluid to detect biologic markers of breast cancer

Sauter, E.R.; Zhu, W.; Fan, X.-J.; Wassell, R.P.; Chervoneva, I.; Du Bois, G.C.
May 2002
British Journal of Cancer;5/6/2002, Vol. 86 Issue 9, p1440
Academic Journal
The early detection of breast cancer is the best means to minimise disease-related mortality. Current screening techniques have limited sensitivity arid specificity. Breast nipple aspirate fluid can be obtained noninvasively and contains proteins secreted from ductal and Iobular epithelia. Nipple aspirate fluid proteins are breast specific and generally more concentrated than corresponding blood levels. Proteomic analysis of I µl of diluted nipple aspirate fluid over a 5-40 kDa range from 20 subjects with breast cancer and 13 with nondiseased breasts identified five differentially expressed proteins. The most sensitive and specific proteins were 6500 and 15 940 Da, found in 75-84% of samples from women with cancer but in only 0-9% of samples from normal women. These findings suggest that (1) differential expression of nipple aspirate fluid proteins exists between women with normal and diseased breasts, and (2) analysis of these proteins may predict the presence of breast cancer.


Related Articles

  • BIOMARKER BEGINS PILOT STUDY OF BREAST CANCER BLOOD TEST.  // Biotech Business;May2005, Vol. 18 Issue 5, p5 

    Reports that Biomarker Technologies LLC has begun a pilot study of the BT Test, a blood diagnostic test for the detection of breast cancer, in the U.S. Key reason for the conduscting the pilot study; Statement of Biomarker CEO William Gartner about BT Test; Number of women diagnosed with breast...

  • miRNA as Potential Biomarkers of Breast Cancer in the Lebanese Population and in Young Women: A Pilot Study. Nassar, Farah J.; El Sabban, Maya; Zgheib, Nathalie K.; Tfayli, Arafat; Boulos, Fouad; Jabbour, Mark; Saghir, Nagi S. E. l.; Talhouk, Rabih; Bazarbachi, Ali; Calin, George A.; Nasr, Rihab // PLoS ONE;Sep2014, Vol. 9 Issue 9, p1 

    Relative to western populations, the percentage of women diagnosed with breast cancer at a young age in Lebanon is high. While the younger age of the Lebanese population compared to the West certainly contributes to this difference, potential genetic, reproductive and/or biological factors...

  • Breast cancer subtyping from plasma proteins. Fan Zhang; Chen, Jake Y. // BMC Medical Genomics;2013, Vol. 6 Issue Suppl 1, p1 

    Background: Early detection of breast cancer in blood is both appealing clinically and challenging technically due to the disease's illusive nature and heterogeneity. Today, even though major breast cancer subtypes have been characterized, i.e., luminal A, luminal B, HER2+, and basal-like,...

  • Targeted Proteomics Enables Simultaneous Quantification of Folate Receptor Isoforms and Potential Isoform-based Diagnosis in Breast Cancer. Yang, Ting; Xu, Feifei; Fang, Danjun; Chen, Yun // Scientific Reports;11/20/2015, p16733 

    The distinct roles of protein isoforms in cancer are becoming increasingly evident. FRα and FRβ, two major isoforms of the folate receptor family, generally have different cellular distribution and tissue specificity. However, the presence of FRβ in breast tumors, where FRα is...

  • University of North Dakota Signs License to Develop Breast Cancer Detection Technology with NeoMatrix LLC, Makers of the Halo(r) Pap Test.  // Biomedical Market Newsletter;1/14/2012, Vol. 21, p1 

    The article presents information on an exclusive option agreement made by University of North Dakota, North Dakota with NeoMatrix LLC in order to develop the HALO(r) breast assay for the early detection of breast cancer. It discusses the use of this assay in screening and assessing breast cancer...

  • Prognostic Significance of Deregulated Dicer Expression in Breast Cancer. Caffrey, Emer; Ingoldsby, Helen; Wall, Deirdre; Webber, Mark; Dinneen, Kate; Murillo, Laura S.; Inderhaug, Celine; Newell, John; Gupta, Sanjeev; Callagy, Grace // PLoS ONE;Dec2013, Vol. 8 Issue 12, p1 

    Background: Dicer, an RNase III-type endonuclease, is the key enzyme involved in RNA interference and microRNA pathways. Aberrant expression of Dicer is reported in several human cancers. Our aim was to assess the prognostic role of Dicer in breast cancer. Methods: The entire series comprised...

  • Nanoelectronic detection of breast cancer biomarker. Chen, Yu; Wang, Xihua; Hong, Mi K.; Rosenberg, Carol L.; Reinhard, Björn M.; Erramilli, Shyamsunder; Mohanty, Pritiraj // Applied Physics Letters;12/6/2010, Vol. 97 Issue 23, p233702 

    There is a fundamental need for semiconductor-based ultrasensitive nanosensors for detecting heterogeneous biomarkers associated with cancer, which do not have a well-defined molecular weight. Here we show that a nanoscale gated biological field effect transistor can be used for detecting the...

  • A Novel Color Change Mechanism for Breast Cancer Biomarker Detection: Naphthoquinones as Specific Ligands of Human Arylamine N-Acetyltransferase 1. Laurieri, Nicola; Egleton, James E.; Varney, Amy; Thinnes, Cyrille C.; Quevedo, Camilo E.; Seden, Peter T.; Thompson, Sam; Rodrigues-Lima, Fernando; Dairou, Julien; Dupret, Jean-Marie; Russell, Angela J.; Sim, Edith // PLoS ONE;Aug2013, Vol. 8 Issue 8, p1 

    Human arylamine N-acetyltransferase 1 (hNAT1) has become an attractive potential biomarker for estrogen-receptor-positive breast cancers. We describe here the mechanism of action of a selective non-covalent colorimetric biosensor for the recognition of hNAT1 and its murine homologue, mNat2, over...

  • An Interobserver Reproducibility Analysis of Ki67 Visual Assessment in Breast Cancer. Shui, Ruohong; Yu, Baohua; Bi, Rui; Yang, Fei; Yang, Wentao // PLoS ONE;May2015, Vol. 10 Issue 5, p1 

    Background: Ki67 labeling index (LI) is used as a predictive marker and is associated with prognosis in breast cancer. However, standardised methodologies for measurement are lacking which has limited its application in clinical practice. In this study, we evaluated the interobserver...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics