TITLE

High Level Ethanol Production by Nitrogen and Osmoprotectant Supplementation under Very High Gravity Fermentation Conditions

AUTHOR(S)
Chan-u-tit, Pachaya; Laopaiboon, Lakkana; Jaisil, Prasit; Laopaiboon, Pattana
PUB. DATE
February 2013
SOURCE
Energies (19961073);Feb2013, Vol. 6 Issue 2, p884
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Optimization of nutrient supplements ie, yeast extract (1, 3 and 5 gL-1), dried spent yeast (DSY: 4, 12 and 20 gL-1) and osmoprotectant (glycine: 1, 3 and 5 gL-1) to improve the efficiency of ethanol production from a synthetic medium under very high gravity (VHG) fermentation by Saccharomyces cerevisiae NP 01 was performed using a statistical method, an L9 (34) orthogonal array design. The synthetic medium contained 280 gL-1 of sucrose as a sole carbon source. When the fermentation was carried out at 30 °C, the ethanol concentration (P), yield (Yp/s) and productivity (Qp) without supplementation were 95.3 gL-1, 0.49 gg-1 and 1.70 gL-1h-1, respectively. According to the orthogonal results, the order of influence on the P and Qp values were yeast extract > glycine > DSY, and the optimum nutrient concentrations were yeast extract, 3; DSY, 4 and glycine, 5 gL-1, respectively. The verification experiment using these parameters found that the P, Yp/s and Qp values were 119.9 gL-1, 0.49 g g-1 and 2.14 gL-1h-1, respectively. These values were not different from those of the synthetic medium supplemented with 9 gL-1 of yeast extract, indicating that DSY could be used to replace some amount of yeast extract. When sweet sorghum juice cv KKU40 containing 280 gL-1 of total sugar supplemented with the three nutrients at the optimum concentrations was used as the ethanol production medium, the P value (1200 gL-1) was not changed, but the Qp value was increased to 250 gL-1h-1.
ACCESSION #
85818424

 

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