TITLE

Induced Pluripotent Stem Cells Show Metabolomic Differences to Embryonic Stem Cells in Polyunsaturated Phosphatidylcholines and Primary Metabolism

AUTHOR(S)
Meissen, John K.; Yuen, Benjamin T. K.; Kind, Tobias; Riggs, John W.; Barupal, Dinesh K.; Knoepfler, Paul S.; Fiehn, Oliver
PUB. DATE
October 2012
SOURCE
PLoS ONE;Oct2012, Vol. 7 Issue 10, Special section p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Induced pluripotent stem cells are different from embryonic stem cells as shown by epigenetic and genomics analyses. Depending on cell types and culture conditions, such genetic alterations can lead to different metabolic phenotypes which may impact replication rates, membrane properties and cell differentiation. We here applied a comprehensive metabolomics strategy incorporating nanoelectrospray ion trap mass spectrometry (MS), gas chromatography-time of flight MS, and hydrophilic interaction- and reversed phase-liquid chromatography-quadrupole time-of-flight MS to examine the metabolome of induced pluripotent stem cells (iPSCs) compared to parental fibroblasts as well as to reference embryonic stem cells (ESCs). With over 250 identified metabolites and a range of structurally unknown compounds, quantitative and statistical metabolome data were mapped onto a metabolite networks describing the metabolic state of iPSCs relative to other cell types. Overall iPSCs exhibited a striking shift metabolically away from parental fibroblasts and toward ESCs, suggestive of near complete metabolic reprogramming. Differences between pluripotent cell types were not observed in carbohydrate or hydroxyl acid metabolism, pentose phosphate pathway metabolites, or free fatty acids. However, significant differences between iPSCs and ESCs were evident in phosphatidylcholine and phosphatidylethanolamine lipid structures, essential and non-essential amino acids, and metabolites involved in polyamine biosynthesis. Together our findings demonstrate that during cellular reprogramming, the metabolome of fibroblasts is also reprogrammed to take on an ESC-like profile, but there are select unique differences apparent in iPSCs. The identified metabolomics signatures of iPSCs and ESCs may have important implications for functional regulation of maintenance and induction of pluripotency.
ACCESSION #
83523025

 

Related Articles

  • Epigenetics of induced pluripotency, the seven-headed dragon. Djuric, Ugljesa; Ellis, James // Stem Cell Research & Therapy;2010, Vol. 1 Issue 1, p1 

    Induction of pluripotency from somatic cells by exogenous transcription factors is made possible by a variety of epigenetic changes that take place during the reprogramming process. The derivation of fully reprogrammed induced pluripotent stem (iPS) cells is achieved through establishment of...

  • Endodermal and Hepatic Differentiation from Human Embryonic Stem Cells and Human Induced Pluripotent Stem Cells. Kawabata, Kenji; Takayama, Kazuo; Nagamoto, Yasuto; Saldon, Mary S.; Higuchi, Maiko; Mizuguchi, Hiroyuki // Journal of Stem Cell Research & Therapy;Special2012, Vol. 2 Issue S, Special section p1 

    Induced hepatocytes differentiated from human embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) have a wide range of potential applications in biomedical research, drug discovery, and the treatment of liver disease. Differentiation of human ESCs and iPSCs into endodermal and...

  • Effect of Different Feeding Schedules on The Survival and Neural Differentiation of Human Embryonic and Induced Pluripotent Stem Cells. JENSEN, Matthew B.; JAGER, Lindsey D.; COHEN, Laura K.; KWOK, Susanna S.; KWON, Jin M.; HALL, Crystal A. // Journal of Neurological Sciences;2014, Vol. 31 Issue 2, p226 

    Neural culture of human pluripotent stem cells is useful for neuroscience research, but the optimal feeding schedule for these in vitro systems is unclear. We evaluated the survival and neural differentiation profiles of human embryonic and induced pluripotent stem cells cultured with medium...

  • Developmental neuroscience: Miniature human brains. Brüstle, Oliver // Nature;9/19/2013, Vol. 501 Issue 7467, p319 

    The article presents a research that examines the effect of induced pluripotent stem (iPS) cells that are generated through the reprogramming of differentiated cells, and embryonic stem (ES) cells as a tool for modelling neurodevelopmental disorders. It determines how iPS cells can help in...

  • Reprogramming in vivo produces teratomas and iPS cells with totipotency features. Abad, María; Mosteiro, Lluc; Pantoja, Cristina; Cañamero, Marta; Rayon, Teresa; Ors, Inmaculada; Graña, Osvaldo; Megías, Diego; Domínguez, Orlando; Martínez, Dolores; Manzanares, Miguel; Ortega, Sagrario; Serrano, Manuel // Nature;10/17/2013, Vol. 502 Issue 7471, p340 

    Reprogramming of adult cells to generate induced pluripotent stem cells (iPS cells) has opened new therapeutic opportunities; however, little is known about the possibility of in vivo reprogramming within tissues. Here we show that transitory induction of the four factors Oct4, Sox2, Klf4 and...

  • INDUCED PLURIPOTENT STEM CELLS: HOW THEY WILL CHANGE THE PRACTICE OF CARDIOVASCULAR MEDICINE. Wing Tak Wong; Sayed, Nazish; Cooke, John P. // Methodist DeBakey Cardiovascular Journal;Oct-Dec2013, Vol. 9 Issue 4, p206 

    Induced pluripotent stem cells (iPSCs) can be generated from adult somatic tissues by the forced expression of a few defined transcription factors, including Oct4, Sox2, Klf4, and c-Myc. iPSC technology holds tremendous promises for therapeutic cardiovascular regeneration because of the cells'...

  • The Application of Human iPSCs in Neurological Diseases: From Bench to Bedside. Xie, Nina; Tang, Beisha // Stem Cells International;1/6/2016, p1 

    In principle, induced pluripotent stem cells (iPSCs) are generated from somatic cells by reprogramming and gaining the capacity to self-renew indefinitely as well as the ability to differentiate into cells of different lineages. Human iPSCs have absolute advantages over human embryonic stem...

  • Proangiogenic Features of Mesenchymal Stem Cells and Their Therapeutic Applications. Tao, Hongyan; Han, Zhibo; Han, Zhong Chao; Li, Zongjin // Stem Cells International;1/6/2016, p1 

    Mesenchymal stem cells (MSCs) have shown their therapeutic potency for treatment of cardiovascular diseases owing to their low immunogenicity, ease of isolation and expansion, and multipotency. As multipotent progenitors, MSCs have revealed their ability to differentiate into various cell types...

  • Embryonic stem cell and induced pluripotent stem cell: an epigenetic perspective. Liang, Gaoyang; Zhang, Yi // Cell Research;Jan2013, Vol. 23 Issue 1, p49 

    Pluripotent stem cells, like embryonic stem cells (ESCs), have specialized epigenetic landscapes, which are important for pluripotency maintenance. Transcription factor-mediated generation of induced pluripotent stem cells (iPSCs) requires global change of somatic cell epigenetic status into an...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics