TITLE

Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage

AUTHOR(S)
Amps, Katherine; Andrews, Peter W; Anyfantis, George; Armstrong, Lyle; Avery, Stuart; Baharvand, Hossein; Baker, Julie; Baker, Duncan; Munoz, Maria B; Beil, Stephen; Benvenisty, Nissim; Ben-Yosef, Dalit; Biancotti, Juan-Carlos; Bosman, Alexis; Brena, Romulo Martin; Brison, Daniel; Caisander, Gunilla; Camarasa, MarĂ­a V; Chen, Jieming; Chiao, Eric
PUB. DATE
December 2011
SOURCE
Nature Biotechnology;Dec2011, Vol. 29 Issue 12, p1132
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. Most lines remained karyotypically normal, but there was a progressive tendency to acquire changes on prolonged culture, commonly affecting chromosomes 1, 12, 17 and 20. DNA methylation patterns changed haphazardly with no link to time in culture. Structural variants, determined from the SNP arrays, also appeared sporadically. No common variants related to culture were observed on chromosomes 1, 12 and 17, but a minimal amplicon in chromosome 20q11.21, including three genes expressed in human ES cells, ID1, BCL2L1 and HM13, occurred in >20% of the lines. Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells.
ACCESSION #
67759340

 

Related Articles

  • Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in rats. Hirabayashi, Masumi; Hochi, Shinichi // Reproductive Medicine & Biology;Dec2011, Vol. 10 Issue 4, p231 

    Over the past 25 years, the reverse genetic approach including precise and conditional replacement or loss of gene function at a specific locus was considered possible only in mice due to the absence of embryonic stem (ES) or induced pluripotent stem (iPS) cell lines in other species. Recently,...

  • Imaging, quantification and visualization of spatio-temporal patterning in mESC colonies under different culture conditions. Scherf, N.; Herberg, M.; Thierbach, K.; Zerjatke, T.; Kalkan, T.; Humphreys, P.; Smith, A.; Glauche, I.; Roeder, I. // Bioinformatics;Sep2012, Vol. 28 Issue 18, pi556 

    Motivation: Mouse embryonic stem cells (mESCs) have developed into a prime system to study the regulation of pluripotency in stable cell lines. It is well recognized that different, established protocols for the maintenance of mESC pluripotency support morphologically and functionally different...

  • NANOG Reporter Cell Lines Generated by Gene Targeting in Human Embryonic Stem Cells. Fischer, Yvonne; Ganic, Elvira; Ameri, Jacqueline; Xiaojie Xian; Johannesson, Martina; Semb, Henrik // PLoS ONE;2010, Vol. 5 Issue 9, p1 

    Background: Pluripotency and self-renewal of human embryonic stem cells (hESCs) is mediated by a complex interplay between extra- and intracellular signaling pathways, which regulate the expression of pluripotency-specific transcription factors. The homeodomain transcription factor NANOG plays a...

  • Advances in understanding the cell types and approaches used for generating induced pluripotent stem cells. Jun Li; Wei Song; Guangjin Pan; Jun Zhou // Journal of Hematology & Oncology;2014, Vol. 7 Issue 1, p1 

    Successfully reprogramming somatic cells to a pluripotent state generates induced pluripotent stem (iPS) cells (or iPSCs), which have extensive self-renewal capacity like embryonic stem cells (ESCs). iPSCs can also generate daughter cells that can further undergo differentiation into various...

  • Development of humanized culture medium with plant-derived serum replacement for human pluripotent stern cells. Kunova, Michaela; Matulka, Kamil; Eiselleova, Livia; Trckova, Petra; Hampl, Ales; Dvorak, Petr // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Nov2010, Vol. 21 Issue 5, p676 

    For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum...

  • Soft Substrate Promotes Pluripotent Stem Cell Culture.  // Trends in Molecular Sciences;2011, Vol. 3 Issue 1, p46 

    The article reports on a study by researchers at the University of Illinois, in which they demonstrated that culturing mouse embryonic stem cells (mESCs) on a soft gel instead of a hard plate or dish keeps them in their pluripotent state. Professor Ning Wang, who co-authored the study, said this...

  • The proteomes of native and induced pluripotent stem cells. Pera, Martin F // Nature Methods;Oct2011, Vol. 8 Issue 10, p807 

    The article focuses on the study by Phanstiel and colleagues on the comparison of induced pluripotent stems cells (iPSCs) and embryonic stem cells (ESCs) on the proteome levels. It states that the comparison of cell lines and multiple samples were facilitated in a single experiment through the...

  • Applying Pluripotent Stem Cells in Drug Discovery. Steger, Matthias // Drug Discovery;2010, Vol. 7, p18 

    There is huge excitement around the potential of pluripotent stem cells in drug discovery. Human embryonic stem cells are proving to be a rich source of previously scarce cell types, such as cardiomyocytes, neurons and hepatocytes, for use in pre-clinical pharmaceutical research. For example, it...

  • Differentiation Potential of O Bombay Human-Induced Pluripotent Stem Cells and Human Embryonic Stem Cells into Fetal Erythroid-Like Cells. Ganji, Fatemeh; Abroun, Saeid; Baharvand, Hossein; Aghdami, Nasser; Ebrahimi, Marzieh // Cell Journal (Yakhteh);Winter2015, Vol. 16 Issue 4, p426 

    Objective: There is constant difficulty in obtaining adequate supplies of blood components, as well as disappointing performance of "universal" red blood cells. Advances in somatic cell reprogramming of human-induced pluripotent stem cells (hiPSCs) have provided a valuable alternative source to...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics