TITLE

Dynamic expression patterns of imprinted genes in human embryonic stem cells following prolonged passaging and differentiation

AUTHOR(S)
Mai, Xiuyun; Mai, Qingyun; Li, Tao; Zhou, Canquan
PUB. DATE
April 2011
SOURCE
Journal of Assisted Reproduction & Genetics;Apr2011, Vol. 28 Issue 4, p315
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: To evaluate the overall expression patterns of imprinted genes in human embryonic stem cells following long term culture and differentiation. Materials and methods: Expression levels of 65 imprinted genes determined by PCR array were analyzed in one human embryonic stem cell line (cHES1) following prolonged passaging and differentiation. Results: Transcripts of 63 imprinted genes were detected in cHES1 cells. Expression levels of all but 5 imprinted genes did not correlate with passage numbers or differ in cells after passage 50 compared with those before passage 50. SLC22A2, SLC22A3, CPA, H19, COPG2IT1 and IGF2 expression were significantly increased in embryoid bodies compared with undifferentiated cells. Conclusions: The global expression profiles of imprinted genes are generally stable in human embryonic stem cells after prolonged passaging and differentiation.
ACCESSION #
61236436

 

Related Articles

  • Comparison of the gene expression profile of undifferentiated human embryonic stem cell lines and differentiating embryoid bodies. Bhattacharya, Bhaskar; Cai, Jingli; Luo, Youngquan; Miura, Takumi; Mejido, Josef; Brimble, Sandii N; Zeng, Xianmin; Schulz, Thomas C; Rao, Mahendra S; Puri, Raj K // BMC Developmental Biology;2005, Vol. 5, p22 

    Background: The identification of molecular pathways of differentiation of embryonic stem cells (hESC) is critical for the development of stem cell based medical therapies. In order to identify biomarkers and potential regulators of the process of differentiation, a high quality microarray...

  • SALL4, a Stem Cell Factor, Affects the Side Population by Regulation of the ATP-Binding Cassette Drug Transport Genes. Ha-Won Jeong; Wei Cui; Youyang Yang; Jiayun Lu; Jie He; Ailing Li; Song, David; Ye Guo; Liu, Bee H.; Li Chai // PLoS ONE;2011, Vol. 6 Issue 4, p1 

    Our previous work shows that the stem cell factor SALL4 plays a central role in embryonic and leukemic stem cells. In this study, we report that SALL4 expression was higher in drug resistant primary acute myeloid leukemic patients than those from drug-responsive cases. In addition, while...

  • Epigenetic status of human embryonic stem cells. Rugg-Gunn, Peter J.; Ferguson-Smith, Anne C.; Pedersen, Roger A. // Nature Genetics;Jun2005, Vol. 37 Issue 6, p585 

    We examined the allele-specific expression of six imprinted genes and the methylation profiles of three imprinting control regions to assess the epigenetic status of human embryonic stem cells. We identified generally monoallelic gene expression and normal methylation patterns. During prolonged...

  • Identification of a novel intronic enhancer responsible for the transcriptional regulation of musashi1 in neural stem/progenitor cells. Kawase, Satoshi; Imai, Takao; Miyauchi-Hara, Chikako; Yaguchi, Kunio; Nishimoto, Yoshinori; Fukami, Shin-ichi; Matsuzaki, Yumi; Miyawaki, Atsushi; Itohara, Shigeyoshi; Okano, Hideyuki // Molecular Brain;2011, Vol. 4 Issue 1, p14 

    Background: The specific genetic regulation of neural primordial cell determination is of great interest in stem cell biology. The Musashi1 (Msi1) protein, which belongs to an evolutionarily conserved family of RNA-binding proteins, is a marker for neural stem/progenitor cells (NS/PCs) in the...

  • Titanium nanotubes stimulate osteoblast differentiation of stem cells from pulp and adipose tissue. Pozio, Alfonso; Palmieri, Annalisa; Girardi, Ambra; Cura, Francesca; Carinci, Francesco // Dental Research Journal;Dec2012, Vol. 9 Issue 8, Supp 2, pS169 

    Background: Titanium is the gold standard among materials used for prosthetic devices because of its good mechanical and chemical properties. When exposed to oxygen, titanium becomes an oxide, anatase that is biocompatible and able to induce osseointegration. Materials and Methods: In this study...

  • Fibroblast behavior after titanium surfaces exposure. Danza, Matteo; Zollino, Ilaria; Candotto, Valentina; Cura, Francesca; Carinci, Francesco // Dental Research Journal;Dec2012, Vol. 9 Issue 8, Supp 2, pS211 

    Background: The main requirements for a good material are its ability to promote attraction and adhesion of bone precursor cells and their proliferation and differentiation. Different biocompatible materials are currently employed as scaffold. Among these, titanium is considered a “gold...

  • Novel cell lines established from pediatric brain tumors. Xu, Jingying; Erdreich-Epstein, Anat; Gonzalez-Gomez, Ignacio; Melendez, Elizabeth; Smbatyan, Goar; Moats, Rex; Rosol, Michael; Biegel, Jaclyn; Reynolds, C. // Journal of Neuro-Oncology;Apr2012, Vol. 107 Issue 2, p269 

    The paucity of cell culture models for childhood brain tumors prompted us to establish pediatric cell lines for use in biological experiments and preclinical developmental therapeutic studies. Three cell lines were established, CHLA-200 (GBM), CHLA-259 (anaplastic medulloblastoma) and CHLA-266...

  • Icariin-mediated expression of cardiac genes and modulation of nitric oxide signaling pathway during differentiation of mouse embryonic stem cells into cardiomyocytes in vitro. Dan-yan Zhu; Yi-jia Lou // Acta Pharmacologica Sinica;Mar2006, Vol. 27 Issue 3, p311 

    Aim: To investigate effects of icariin on cardiac gene expression and the modulation of nitric oxide (NO) signal transduction during the differentiation of embryonic stem (ES) cells into cardiomyocytes in vitro. Methods: The expression levels of cardiac developmental-dependent genes were...

  • Genome-wide gene expression profiling of testicular carcinoma in situ progression into overt tumours. Almstrup, K.; Hoei-Hansen, C. E.; Nielsen, J. E.; Wirkner, U.; Ansorge, W.; Skakkebæk, N. E.; Rajpert-De Meyts, E.; Leffers, H. // British Journal of Cancer;5/23/2005, Vol. 92 Issue 10, p1934 

    The carcinoma in situ (CIS) cell is the common precursor of nearly all testicular germ cell tumours (TGCT). In a previous study, we examined the gene expression profile of CIS cells and found many features common to embryonic stem cells indicating that initiation of neoplastic transformation...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics