TITLE

Agouti C57BL/6N embryonic stem cells for mouse genetic resources

AUTHOR(S)
Pettitt, Stephen J.; Qi Liang; Rairdan, Xin Y.; Moran, Jennifer L.; Prosser, Haydn M.; Beier, David R.; Lloyd, Kent C.; Bradley, Allan; Skarnes, William C.
PUB. DATE
July 2009
SOURCE
Nature Methods;Jul2009, Vol. 6 Issue 7, p493
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
ACCESSION #
43750031

 

Related Articles

  • Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Amps, Katherine; Andrews, Peter W; Anyfantis, George; Armstrong, Lyle; Avery, Stuart; Baharvand, Hossein; Baker, Julie; Baker, Duncan; Munoz, Maria B; Beil, Stephen; Benvenisty, Nissim; Ben-Yosef, Dalit; Biancotti, Juan-Carlos; Bosman, Alexis; Brena, Romulo Martin; Brison, Daniel; Caisander, Gunilla; Camarasa, María V; Chen, Jieming; Chiao, Eric // Nature Biotechnology;Dec2011, Vol. 29 Issue 12, p1132 

    The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide...

  • Hematopoietic Development from Human Induced Pluripotent Stem Cells. Lengerke, Claudia; Grauer, Matthias; Niebuhr, Nina I.; Riedt, Tamara; Kanz, Lothar; Park, In‐Hyun; Daley, George Q. // Annals of the New York Academy of Sciences;Sep2009, Vol. 1176 Issue 1, p219 

    A decade of research on human embryonic stem cells (ESC) has paved the way for the discovery of alternative approaches to generating pluripotent stem cells. Combinatorial overexpression of a limited number of proteins linked to pluripotency in ESC was recently found to reprogram differentiated...

  • Epigenetic status of human embryonic stem cells. Rugg-Gunn, Peter J.; Ferguson-Smith, Anne C.; Pedersen, Roger A. // Nature Genetics;Jun2005, Vol. 37 Issue 6, p585 

    We examined the allele-specific expression of six imprinted genes and the methylation profiles of three imprinting control regions to assess the epigenetic status of human embryonic stem cells. We identified generally monoallelic gene expression and normal methylation patterns. During prolonged...

  • Identification of a novel intronic enhancer responsible for the transcriptional regulation of musashi1 in neural stem/progenitor cells. Kawase, Satoshi; Imai, Takao; Miyauchi-Hara, Chikako; Yaguchi, Kunio; Nishimoto, Yoshinori; Fukami, Shin-ichi; Matsuzaki, Yumi; Miyawaki, Atsushi; Itohara, Shigeyoshi; Okano, Hideyuki // Molecular Brain;2011, Vol. 4 Issue 1, p14 

    Background: The specific genetic regulation of neural primordial cell determination is of great interest in stem cell biology. The Musashi1 (Msi1) protein, which belongs to an evolutionarily conserved family of RNA-binding proteins, is a marker for neural stem/progenitor cells (NS/PCs) in the...

  • Feeding hungry stem cells. Pedersen, Roger A. // Nature Biotechnology;Sep2002, Vol. 20 Issue 9, p882 

    Reports on research by M. Richards and colleagues demonstrating that two pre-existing human embryonic stem (HES) cell lines retained their undifferentiated state when cultured with human fetal fibroblasts. Theoretical and practical challenges posed by the dependence of HES cells on any feeder...

  • Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Hotta, Akitsu; Cheung, Aaron Y. L.; Farra, Natalie; Vijayaragavan, Kausalia; Séguin, Cheryle A.; Draper, Jonathan S.; Pasceri, Peter; Maksakova, Irina A.; Mager, Dixie L.; Rossant, Janet; Bhatia, Mickie; Ellis, James // Nature Methods;May2009, Vol. 6 Issue 5, p370 

    Induced pluripotent stem (iPS) cells may be of use in regenerative medicine. However, the low efficiency of reprogramming is a major impediment to the generation of patient-specific iPS cell lines. Here we report the first selection system for the isolation of human iPS cells. We developed the...

  • Genomic instability of human embryonic stem cell lines using different passaging culture methods. Tosca, Lucie; Feraud, Olivier; Magniez, Aurélie; Bas, Cécile; Griscelli, Frank; Bennaceur-Griscelli, Annelise; Tachdjian, Gérard // Molecular Cytogenetics (17558166);2015, Vol. 8 Issue 1, p1 

    Background: Human embryonic stem cells exhibit genomic instability that can be related to culture duration or to the passaging methods used for cell dissociation. In order to study the impact of cell dissociation techniques on human embryonic stem cells genomic instability, we cultured H1 and H9...

  • Novel lines of Pax6-I- embryonic stem cells exhibit reduced neurogenic capacity without loss of viability. Quinn, Jane C; Molinek, Michael; Nowakowski, Tomasz J.; Mason, John O.; Price, David J. // BMC Neuroscience;2010, Vol. 11, p26 

    Background: Embryonic stem (ES) cells can differentiate into all cell types and have been used extensively to study factors affecting neuronal differentiation. ES cells containing mutations in known genes have the potential to provide useful in vitro models for the study of gene function during...

  • New Human Embryonic Stem-Cell Lines to Be Made Available to Researchers.  // Ascribe Newswire: Medicine;3/4/2004, p20 

    Howard Hughes Medical Institute researchers at Harvard University announced today that they have derived 17 new human embryonic stem-cell lines. The new cell lines will be made available to researchers, although at this time United States policies prohibit the use of federal funds to investigate...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics