TITLE

Oocyte cryopreservation: the birth of the first Hungarian babies from frozen oocytes

AUTHOR(S)
Konc, Janos; Kanyo, Katalin; Varga, Erika; Kriston, Rita; Cseh, Sandor
PUB. DATE
July 2008
SOURCE
Journal of Assisted Reproduction & Genetics;Jul2008, Vol. 25 Issue 7, p349
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose To present data obtained with clinical application of oocyte cryopreservation. Methods Slow freezing/rapid thawing in PBS based medium containing 1.5 M propanediol+0.3 M sucrose. Results A total of 127 embryos were transferred into 54 patients (1.9 embryo/cycle, 64 transfer cycles). Clinical pregnancy rate of 20% per cycle (13/64) and 24.0% per patient were achieved. Up-to-date, six patients delivered seven healthy babies; there are four ongoing pregnancies. Three abortions (23%) and one biochemical pregnancy (0.7%) was obtained. Implantation rates of 11% per transferred embryos (14/127) and 6.5% (14/215) per thawed eggs were found. In each case, normal karyotype was detected. No difference was found in the ratio of spindle positive oocytes at the polscope analyses done before and after freezing (75.8% vs. 82.5%). Conclusion Egg freezing is not a routine procedure yet, but there will certainly be a place for it in the future of assisted reproductive medicine.
ACCESSION #
34462582

 

Related Articles

  • Five-Year Experience with a Sodium-Depleted Slow-Freezing Method.  // Fertility Weekly;10/30/2006, p7 

    The article presents a study that examined the effectiveness of oocyte cryopreservation by the use of sodium-depleted freezing media. Data used came from 53 frozen egg-embryo transfer cycles in 46 patients that were carried from April 2000 to August 2005. Patients underwent fresh non-donor...

  • Mammalian Oocyte Cryopreservation - Review. Cean, Ada; Bogdan, Alexandru T.; Pacala, Nicolae; Ivan, Alexandra; Ilie, Daniela E. // Scientific Papers: Animal Science & Biotechnologies / Lucrari St;Jun2011, Vol. 44 Issue 1, p370 

    Although oocyte cryopreservation represents one of the main objectives of the reproductive medicine and cryobiology in the last years, until now, regardless of the studied specie, there is no freezing protocol that will assure satisfactory survival rates after thawing. Oocyte cryopreservation is...

  • Effects of sucrose concentration on the developmental potential of human frozen–thawed oocytes at different stages of maturity. Z.J. Chen; M. Li; Y. Li; L.X. Zhao; R. Tang; Y. Sheng; X. Gao; C.H. Chang; H.L. Feng // Human Reproduction;Oct2004, Vol. 19 Issue 10, p2345 

    BACKGROUD: Success of human oocyte cryopreservation depends on multiple cryobiological factors that could influence the developmental potential of the oocytes. The objective of this study was to examine the effects of different sucrose concentrations on the developmental potential of human...

  • Freezing within 2 h from oocyte retrieval increases the efficiency of human oocyte cryopreservation when using a slow freezing/rapid thawing protocol with high sucrose concentration. L. Parmegiani; G.E. Cognigni; S. Bernardi; W. Ciampaglia; F. Infante; P. Pocognoli; C. Tabarelli de Fatis; E. Troilo; M. Filicori // Human Reproduction;Aug2008, Vol. 23 Issue 8, p1771 

    BACKGROUND A number of factors influence the success of oocyte cryopreservation and subsequent ICSI. The aim of the present study is to establish the ideal time, after oocyte retrieval, for human oocyte cryopreservation via slow freezing/rapid thawing protocol with 0.3 M sucrose concentration in...

  • Little consensus on egg freezing. Maher, Brendan // Nature;10/25/2007, Vol. 449 Issue 7165, p958 

    The article focuses on the scientific issues surrounding the practice of egg freezing for women facing fertility treatment in the U.S. According to a findings presented during the annual meeting of the American Society for Reproductive Medicine (ASRM) in Washington D.C., egg freezing does not...

  • Agentes crioprotetores intracelulares: características e utilização na criopreservação de tecido ovariano e oócitos. Castro, Simone Vieira; de Andrade Carvalho, Adeline; Gomes da Silva, Cleidson Manoel; Rocha Faustino, Luciana; de Figueiredo, José Ricardo; Ribeiro Rodrigues, Ana Paula // Acta Scientiae Veterinariae;Mar2011, Vol. 39 Issue 2, Special section p1 

    Background: The application of cryopreservation in human and animal reproductive medicine has stimulated several studies about the effects of low temperatures and freezing processes on cells and tissues, in order to develop efficient protocols for gamete and embryo preservation. Moreover,...

  • OPTIMUM FOR CRYOPRESERVATION OF HUMAN OVARIAN TISSUE: GENE EXPRESSION AS A DETECTOR OF EFFECTIVENESS. Isachenko, V.; Lapidus, I.; Isachenko, E.; Hancke, K.; Nawroth, F.; Wiegratz, I.; Kreienberg, R. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Oct2010 S3 Supplemen, Vol. 20, pS54 

    Objective: Cryopreservation, the most important stage of the cryobanking of ovarian tissue, can be carried out using one of two methods: conventional ("slow") freezing, and vitrification (direct plunging into liquid nitrogen). For human oocytes vitrification is more effective compared to...

  • History of oocyte cryopreservation. Gook, Debra A. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Sep2011, Vol. 23 Issue 3, p281 

    The potential advantages of being able to cryopreserve oocytes have been apparent for many decades. Technical difficulties associated with the unique properties of the mammalian oocyte initially retarded rapid development in this area but recent advances have overcome many of the problems. A...

  • Objective evaluation of the viability of cryopreserved oocytes. De Santis, L.; Cino, I.; Coticchio, G.; Fusi, F. M.; Papaleo, E.; Rabellotti, E.; Brigante, C.; Borini, A.; Ferrari, A. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Sep2007, Vol. 15 Issue 3, p338 

    Recent studies of fundamental cryobiology, empirical observations and more systematic clinical experiences have generated a renewed interest in oocyte cryopreservation. Poor survival rate has long been the limiting factor which has prevented widespread adoption of oocyte storage. Slow-cooling...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics