TITLE

Generation, Culture, and Differentiation of Human Embryonic Stem Cells for Therapeutic Applications

AUTHOR(S)
Shin Yong Moon; Yong Bin Park; Dae-Sung Kim; Sun Kyung Oh; Dong-Wook Kim
PUB. DATE
January 2006
SOURCE
Molecular Therapy;Jan2006, Vol. 13 Issue 1, p5
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Embryonic stem (ES) cells, derived from the inner cell mass of the mammalian blastocyst, can continuously proliferate in an undifferentiated state and can also be induced to differentiate into a desired cell lineage. These abilities make ES cells an appealing source for cell replacement therapies, the study of developmental biology, and drug/toxin screening studies. As compared to mouse ES cells, human ES cells have only recently been derived and studied. Although there are many differences in properties between mouse and human ES cells, the study of mouse ES cells has provided important insights into human ES cell research. In this review, we describe the advantages and disadvantages of methods used for human ES cell derivation, the expansion of human ES cells, and the current status of human ES cell differentiation research. In addition, we discuss the endeavor that scientists have undertaken toward the therapeutic application of these cells, which includes therapeutic cloning and the improvement of human ES cell culture conditions.Molecular Therapy (2006) 13, 5–14; doi: 10.1016/j.ymthe.2005.09.008
ACCESSION #
25973091

 

Related Articles

  • Joys match fears as California agrees to stem-cell proposal. Knight, Jonathan // Nature;11/11/2004, Vol. 432 Issue 7014, p135 

    The article presents a report on human embryonic stem-cell research in California. A $3-billion programme that intends to make California a world centre for human embryonic stem-cell research got the green light from voters on November 2, but some fear repercussions. The measure was conceived by...

  • Stem cell culture, one step at a time. Pera, Martin F. // Nature Methods;Mar2005, Vol. 2 Issue 3, p164 

    Focuses on the relevance of the derivation of human embryonic stem cells in the development of a defined system for human embryonic stem cell culture free of animal products. Impact of the introduction of inhibitors of bone morphogenic proteins along with basic fibroblast growth factor into...

  • Virgin birth method could found stem cell dynasties. Pag´n Westphal, Sylvia // New Scientist;4/26/2003, Vol. 178 Issue 2392, p17 

    The article reveals the phenomenon that leads to “virgin births” in some species who are a source of embryonic stem cells.Researchers are obtaining human stem cells this way for the first time, and animal experiments suggest such cells are indistinguishable from normal stem cells. So...

  • An overview and synopsis of techniques for directing stem cell differentiation in vitro. Boon Chin Heng; Tong Cao; Haider, Husnain Khawaja; Dennis Zheng Ming Wang; Eugene Kwang-Wei Sim; Soon Chye Ng // Cell & Tissue Research;Mar2004, Vol. 315 Issue 3, p291 

    The majority of studies on stem cell differentiation have so far been based in vivo, on live animal models. The usefulness of such models is limited, since it is much more technically challenging to conduct molecular studies and genetic manipulation on live animal models compared to in vitro...

  • Human Embryonic Stem Cell Research: Structuring Oversight in International Collaborations. BHAN, ANANT; HONGKUI DENG; LORING, JEANNE F.; MORENO, JONATHAN D.; MING YIN; XIAOMEI ZHAI; LAVERY, JAMES V. // Brown Journal of World Affairs;Fall/Winter2008, Vol. 15 Issue 1, p145 

    The article provides an overview of the Chinese guidelines and current U.S. guidelines for human embryonic stem cell (hESC) research. The direction of China's research program is toward clinical applications of stem cells, such as the use of stem cell therapies for leukemia and myocardial...

  • Can science resolve the ethical impasse in stem cell research? Snyder, Evan Y.; Hinman, Lawrence M.; Kalichman, Michael W. // Nature Biotechnology;Apr2006, Vol. 24 Issue 4, p397 

    The article comments on the ethical deadlock in stem cell research. Approaches in the understanding of the control of early human embryonic development could extend solutions to the moral dilemmas affiliated with human embryonic stem cell research, the author stressed. He added that the issue...

  • Human embryonic stem cells flock together. Lensch, M. William; Daley, George Q. // Nature Biotechnology;Jul2007, Vol. 25 Issue 7, p748 

    The article offers information about the comparative study of a large and diverse set of human embryonic stem cell lines (hES) derived and maintained in different laboratories worldwide. It is noted that the research showed that despite unique genetic backgrounds, derivation in various...

  • Clonal tracking of hESCs reveals differential contribution to functional assays. Stewart, Morag H.; Bendall, Sean C.; Levadoux-Martin, Marilyne; Bhatia, Mickie // Nature Methods;Nov2010, Vol. 7 Issue 11, p917 

    Human embryonic stem cells (hESCs) have unique self-renewal and differentiation properties, which are experimentally measured using functional assays. hESC cultures are known to be heterogenous, but whether subsets of cells contribute differently to functional assays has yet to be examined....

  • Beyond clones.  // New Scientist;2/21/2004, Vol. 181 Issue 2435, p3 

    There are varied reactions to the new from South Korea on cloning human embryos. Some have welcomed the study as the breakthrough that will finally unlock the awesome medical potential of embryonic stem cells. Others are outraged, arguing that it will encourage reproductive cloning and lead to a...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics