TITLE

Acid phosphatase production by recombinant Arxula adeninivorans

AUTHOR(S)
Minocha, V.; Kaur, Parvinder; Satyanarayana, T.; Kunze, G.
PUB. DATE
August 2007
SOURCE
Applied Microbiology & Biotechnology;Aug2007, Vol. 76 Issue 2, p387
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Acid phosphatase production by recombinant Arxula adeninivorans was carried out in submerged fermentation. Using the Plackett–Burman design, three fermentation variables (pH, sucrose concentration, and peptone concentration) were identified to significantly affect acid phosphatase and biomass production, and these were optimized using response surface methodology of central composite design. The highest enzyme yields were attained in the medium with 3.9% sucrose and 1.6% peptone at pH 3.8. Because of optimization, 3.86- and 4.19-fold enhancement in enzyme production was achieved in shake flasks (17,054 U g−1 DYB) and laboratory fermenter (18,465 U g−1 DYB), respectively.
ACCESSION #
25882787

 

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