TITLE

Profilin2 contributes to synaptic vesicle exocytosis, neuronal excitability, and novelty-seeking behavior

AUTHOR(S)
Pilo Boyl, Pietro; Di Nardo, Alessia; Mulle, Christophe; Sassoè-Pognetto, Marco; Panzanelli, Patrizia; Mele, Andrea; Kneussel, Matthias; Costantini, Vivian; Perlas, Emerald; Massimi, Marzia; Vara, Hugo; Giustetto, Maurizio; Witke, Walter
PUB. DATE
June 2007
SOURCE
EMBO Journal;6/20/2007, Vol. 26 Issue 12, p2991
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Profilins are actin binding proteins essential for regulating cytoskeletal dynamics, however, their function in the mammalian nervous system is unknown. Here, we provide evidence that in mouse brain profilin1 and profilin2 have distinct roles in regulating synaptic actin polymerization with profilin2 preferring a WAVE-complex-mediated pathway. Mice lacking profilin2 show a block in synaptic actin polymerization in response to depolarization, which is accompanied by increased synaptic excitability of glutamatergic neurons due to higher vesicle exocytosis. These alterations in neurotransmitter release correlate with a hyperactivation of the striatum and enhanced novelty-seeking behavior in profilin2 mutant mice. Our results highlight a novel, profilin2-dependent pathway, regulating synaptic physiology, neuronal excitability, and complex behavior.
ACCESSION #
25463547

 

Related Articles

  • T-type channels-secretion coupling: evidence for a fast low-threshold exocytosis. Carbone, E.; Marcantoni, A.; Giancippoli, A.; Guido, D.; Carabelli, V. // Pflugers Archiv European Journal of Physiology;Dec2006, Vol. 453 Issue 3, p373 

    T-type channels are transient low-voltage-activated (LVA) Ca2+ channels that control Ca2+ entry in excitable cells during small depolarizations around resting potential. Studies in the past 20 years focused on the biophysical, physiological, and molecular characterization of T-type channels in...

  • Real-time measurements of vesicle-SNARE recycling in synapses of the central nervous system. Sankaranarayanan, Sethuraman; Ryan, Timothy A. // Nature Cell Biology;Apr2000, Vol. 2 Issue 4, p197 

    Following the fusion of synaptic vesicles with the presynaptic plasma membrane of nerve terminals by the process of exocytosis, synaptic-vesicle components are recycled to replenish the vesicle pool. Here we use a pH-sensitive green fluorescent protein to measure the residence time of VAMP, a...

  • Polymeric elements for adaptive networks. Erokhin, V. V.; Berzina, T. S.; Fontana, M. P. // Crystallography Reports;Jan2007, Vol. 52 Issue 1, p159 

    Polymeric electrochemical elements of adaptive networks are considered. The main features of these elements are compared with the elements of the nervous system of the snail Lymnaea stagnalis. In particular, synthetic analogues of neurons and synapses are fabricated. The capability of the system...

  • Structural and Mutational Analysis of Functional Differentiation between Synaptotagmins-1 and -7. Mingshan Xue; Craig, Timothy K.; Ok-Ho Shin; Liyi Li; Brautigam, Chad A.; Tomchick, Diana R.; Südhof, Thomas C.; Rosenmund, Christian; Rizo, Josep // PLoS ONE;2010, Vol. 5 Issue 9, p1 

    Synaptotagmins are known to mediate diverse forms of Ca2+-triggered exocytosis through their C2 domains, but the principles underlying functional differentiation among them are unclear. Synaptotagmin-1 functions as a Ca2+ sensor in neurotransmitter release at central nervous system synapses, but...

  • N-methyl-D-aspartate receptor signaling results in Aurora kinase-catalyzed CPEB phosphorylation and aCaMKII mRNA polyadenylation at synapses. Yi-Shuian Huang; Mi-Young Jung; Sarkissian, Madathia; Richter, Joel D. // EMBO Journal;5/1/2002, Vol. 21 Issue 9, p2139 

    Activity-dependent local translation of dendritic mRNAs is one process that underlies synaptic plasticity. Here, we demonstrate that several of the factors known to control polyadenylation-induced translation in early vertebrate development [cytoplasmic polyadenylation element-binding protein...

  • Myosin VI targeting to clathrin-coated structures and dimerization is mediated by binding to Disabled-2 and PtdIns(4,5)P2. Spudich, Giulietta; Chibalina, Margarita V.; Au, Josephine Sui-Yan; Arden, Susan D.; Buss, Folma; Kendrick-Jones, John // Nature Cell Biology;Feb2007, Vol. 9 Issue 2, p176 

    Vesicle transport is essential for the movement of proteins, lipids and other molecules between membrane compartments within the cell. The role of the class VI myosins in vesicular transport is particularly intriguing because they are the only class that has been shown to move 'backwards'...

  • Temporal and spatial coordination of exocytosis and endocytosis. Gundelfinger, Eckart D.; Kessels, Michael M.; Qualmann, Britta // Nature Reviews Molecular Cell Biology;Feb2003, Vol. 4 Issue 2, p127 

    In secretory cells, exocytosis and compensatory endocytosis are tightly coupled membrane trafficking processes that control the surface area and composition of the plasma membrane. While exocytic and endocytic processes have been studied independently in great detail, at present there is much...

  • Parvalbumin-containing neurons mediate the feedforward inhibition of rat rubrospinal neurons. Liu, Chuen-Lan; Wang, Yueh-Jan; Chen, Jeng-Rung; Tseng, Guo-Fang // Anatomy & Embryology;Jun2002, Vol. 205 Issue 3, p245 

    The calcium-binding protein, parvalbumin and glutamic acid decarboxylase immunohistochemistry were used to locate candidate neurons mediating the inhibition of rat rubral neurons. A group of cells with small to medium-sized cell bodies that reacted positively to both were found in the red...

  • Many faces of drebrin: from building dendritic spines and stabilizing gap junctions to shaping neurite-like cell processes. Majoul, Irina; Shirao, Tomoaki; Sekino, Yuko; Duden, Rainer // Histochemistry & Cell Biology;Apr2007, Vol. 127 Issue 4, p355 

    In this review we consider the multiple functions of developmentally regulated brain protein (drebrin), an actin-binding protein, in the formation of cellular polarity in different cell types. Drebrin has a well-established role in the morphogenesis, patterning and maintenance of dendritic...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics