Transcriptional Responses of Mycobacterium tuberculosis Exposed to Adverse Conditions In Vitro

Bacon, Joanna; Marsh, Philip D.
May 2007
Current Molecular Medicine;May2007, Vol. 7 Issue 3, p277
Academic Journal
Mycobacterium tuberculosis encounters a range of stimuli in the host. Understanding the environmental cues that initiate the transcriptional response of M. tuberculosis, which enable the bacterium to replicate and/or survive in the host, will provide markers that are specific to different stages of disease, further refining the search for improved treatments and vaccines. Studying M. tuberculosis gene expression in vivo is technically challenging and more amenable in vitro experiments are being used to aid interpretation and to dissect the signals that are responsible for controlling subsets of genes. Key parameters that affect the growth of a pathogen in the host include nutrient status, environmental pH, oxygen availability, and host defences. Studying gene expression, pathogenicity, and physiology of M. tuberculosis that has been exposed to these relevant host conditions in vitro will further increase our understanding of the virulence factors that M. tuberculosis requires to establish disease. Complementary information obtained by metabolic flux analysis, proteomics, and regulatory networks analysis will enable a clearer picture of how transcriptional responses translate to changes in the metabolome and physiology of the organism.


Related Articles

  • Coadministration of Interleukin 2 Plasmid DNA with Combined DNA Vaccines Significantly Enhances the Protective Efficacy Against Mycobacterium tuberculosis. Cai, H.; Yu, D. H.; Tian, X.; Zhu, Y. X. // DNA & Cell Biology;Oct2005, Vol. 24 Issue 10, p605 

    Coadministration of interleukin 2(IL-2) plasmid DNA with combined DNA vaccines enhanced Th1-type cellular responses by producing higher amounts of IFN-γ with a higher ratio of antigen-specific IgG2a/IgG1. The IFN-γ specific for Ag85B, MPT64, and MPT83 in this group was 415, 267, and...

  • Comparative analysis and assessment of M. tuberculosis H37Rv protein-protein interaction datasets. Zhou, Hufeng; Wong, Limsoon // BMC Genomics;2011 Supplement 3, Vol. 12 Issue Suppl 3, p1 

    Background: M. tuberculosis is a formidable bacterial pathogen. There is thus an increasing demand on understanding the function and relationship of proteins in various strains of M. tuberculosis. Protein-protein interactions (PPIs) data are crucial for this kind of knowledge. However, the...

  • Evaluating the Sensitivity of Mycobacterium tuberculosis to Biotin Deprivation Using Regulated Gene Expression. Sae Woong Park; Klotzsche, Marcus; Wilson, Daniel J.; Boshoff, Helena I.; Hyungjin Eoh; Manjunatha, Ujjini; Blumenthal, Antje; Kyu Rhee; Barry III, Clifton E.; Aldrich, Courtney C.; Ehrt, Sabine; Schnappinger, Dirk // PLoS Pathogens;Sep2011, Vol. 7 Issue 9, Special section p1 

    In the search for new drug targets, we evaluated the biotin synthetic pathway of Mycobacterium tuberculosis (Mtb) and constructed an Mtb mutant lacking the biotin biosynthetic enzyme 7,8-diaminopelargonic acid synthase, BioA. In biotin-free synthetic media, ΔbioA did not produce wild-type...

  • Effects of infection and disease with Mycobacterium tuberculosis on serum antibody to glucan and arabinomannan: two surface polysaccharides of this pathogen. Keitel, Wendy A.; Dai, ZhongDong; Awe, Robert W.; Atmar, Robert L.; Morris, Sheldon; Schneerson, Rachel; Robbins, John B. // BMC Infectious Diseases;2013, Vol. 13 Issue 1, p1 

    Background: The role of the surface capsular polysaccharides (CPs) of Mycobacterium tuberculosis (Mtb) in the pathogenesis of infection and disease, as well their potential for use as diagnostic reagents and vaccine antigens, are unknown. Methods: Serum antibody to two CPs of Mtb, arabinomannan...

  • DNA Methylation Impacts Gene Expression and Ensures Hypoxic Survival of Mycobacterium tuberculosis. Shell, Scarlet S.; Prestwich, Erin G.; Baek, Seung-Hun; Shah, Rupal R.; Sassetti, Christopher M.; Dedon, Peter C.; Fortune, Sarah M. // PLoS Pathogens;Jul2013, Vol. 9 Issue 7, p1 

    DNA methylation regulates gene expression in many organisms. In eukaryotes, DNA methylation is associated with gene repression, while it exerts both activating and repressive effects in the Proteobacteria through largely locus-specific mechanisms. Here, we identify a critical DNA...

  • The Stringent Response Is Required for Full Virulence of Mycobacterium tuberculosis in Guinea Pigs. Klinkenberg, Lee G.; Jong-Hee Lee; Bishai, William R.; Karakousis, Petros C. // Journal of Infectious Diseases;11/1/2010, Vol. 202 Issue 9, p1397 

    During human latent tuberculosis infection, Mycobacterium tuberculosis likely resides within the nutrient-starved environment of caseous lung granulomas. The stringent response alarmone (p)ppGpp is synthesized by Rel in response to nutrient starvation, thus enabling tubercle bacilli to restrict...

  • Bacterial cold shock responses. Weber, Michael H. W.; Marahiel, Mohamed A. // Science Progress;Feb2003, Vol. 86 Issue 1/2, p9 

    As a measure for molecular motion, temperature is one of the most important environmental factors for life as it directly influences structural and hence functional properties of cellular components. After a sudden increase in ambient temperature, which is termed heat shock, bacteria respond by...

  • A Temperature-Responsive Network Links Cell Shape and Virulence Traits in a Primary Fungal Pathogen. Beyhan, Sinem; Gutierrez, Matias; Voorhies, Mark; Sil, Anita // PLoS Biology;Jul2013, Vol. 11 Issue 7, p1 

    : Analysis of a transcriptional regulatory network in a fungal pathogen reveals that four interdependent transcription factors respond to human body temperature to trigger changes in cell shape and virulence gene expression.

  • Antimicrobial Peptides from Fish. Masso-Silva, Jorge A.; Diamond, Gill // Pharmaceuticals;Mar2014, Vol. 7 Issue 3, p265 

    Antimicrobial peptides (AMPs) are found widely distributed through Nature, and participate in the innate host defense of each species. Fish are a great source of these peptides, as they express all of the major classes of AMPs, including defensins, cathelicidins, hepcidins, histone-derived...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics