TITLE

A common lipid links Mfn-mediated mitochondrial fusion and SNARE-regulated exocytosis

AUTHOR(S)
Seok-Yong Choi; Ping Huang; Jenkins, Gary M.; Chan, David C.; Schiller, Juergen; Frohman, Michael A.
PUB. DATE
November 2006
SOURCE
Nature Cell Biology;Nov2006, Vol. 8 Issue 11, p1255
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Fusion of vesicles into target membranes during many types of regulated exocytosis requires both SNARE-complex proteins and fusogenic lipids, such as phosphatidic acid. Mitochondrial fusion is less well understood but distinct, as it is mediated instead by the protein Mitofusin (Mfn). Here, we identify an ancestral member of the phospholipase D (PLD) superfamily of lipid-modifying enzymes that is required for mitochondrial fusion. Mitochondrial PLD (MitoPLD) targets to the external face of mitochondria and promotes trans-mitochondrial membrane adherence in a Mfn-dependent manner by hydrolysing cardiolipin to generate phosphatidic acid. These findings reveal that although mitochondrial fusion and regulated exocytic fusion are mediated by distinct sets of protein machinery, the underlying processes are unexpectedly linked by the generation of a common fusogenic lipid. Moreover, our findings suggest a novel basis for the mitochondrial fragmentation observed during apoptosis.
ACCESSION #
22935823

 

Related Articles

  • Patch amperometry: high-resolution measurements of single-vesicle fusion and release. Dernick, Gregor; Liang-Wei Gong; Tabares, Lucia; de Toledo, Guillermo Alvarez; Lindau, Manfred // Nature Methods;Sep2005, Vol. 2 Issue 9, p699 

    Provides information on a technique for the observation of single-vesicle exocytosis called patch amperometry. Procedure for the detection of exocytosis; Provision of the membrane capacitance by the admittance measurement; Instruction for method application.

  • Clamping down on exocytosis. Rowan, Alison // Nature Reviews Neuroscience;Aug2006, Vol. 7 Issue 8, p593 

    The article discusses research being done on the role of complexin, along with exocytotic SNARE proteins, in calcium-mediated vesicle fusion. It references a study by C. G. Giraudo in the June 22, 2006 issue of "Science." Synaptotagmin, when added on its own or in the presence of calcium, had...

  • Molecular determinants of exocytosis. Bruns, Dieter; Jahn, Reinhard // Pflugers Archiv European Journal of Physiology;Jan2002, Vol. 443 Issue 3, p333 

    Membrane fusion processes occur throughout the cell. Among those, exocytosis of secretory organelles is probably one of the fastest fusion events animal cells can achieve. Structural and functional studies have provided a conceptual framework and a starting point for our mechanistic...

  • TOM-independent complex formation of Bax and Bak in mammalian mitochondria during TNFα-induced apoptosis. Ross, K.; Rudel, T.; Kozjak-Pavlovic, V. // Cell Death & Differentiation;May2009, Vol. 16 Issue 5, p697 

    The Bcl-2 family proteins Bax and Bak are activated in response to many apoptotic stimuli. As a consequence of activation, Bax and Bak oligomerize and permeabilize the outer mitochondrial membrane to permit the release of apoptosis-inducing factors. It still remains unclear whether these...

  • Regulated targeting of Bax and Bak to intracellular membranes during apoptosis. Heath-Engel, H. M.; Shore, G. C. // Cell Death & Differentiation;Aug2006, Vol. 13 Issue 8, p1277 

    The article focuses on the complex regulation of the Bax and Bak death effectors of cells. The pro survival of Bcl-2 proteins provides connections and prevention to oligomerization of Bax and Bak, either through constitutive interactions or following conformational changes in Bax or in Bak....

  • Evolving nature of the AP2a-appendage hub during clathrin-coated vesicle endocytosis. Praefcke, Gerrit J. K.; Ford, Marijn G. J.; Schmid, Eva M.; Olesen, Lene E.; Gallop, Jennifer L.; Peak-Chew, Sew-Yeu; Vallis, Yvonne; Babu, M. Madan; Mills, Ian G.; McMahon, Harvey T. // EMBO Journal;11/10/2004, Vol. 23 Issue 22, p4371 

    Clathrin-mediated endocytosis involves the assembly of a network of proteins that select cargo, modify membrane shape and drive invagination, vesicle scission and uncoating. This network is initially assembled around adaptor protein (AP) appendage domains, which are protein interaction hubs....

  • Exocytosis. Jahn, R.; Lang, T. // Encyclopedic Reference of Molecular Pharmacology;2004, p357 

    An encyclopedia entry for exocytosis is presented. It refers to cellular secretion from (macro)-molecules mediated by fusion of vesicles with the plasma membrane. The basic mechanisms of exocytosis are discussed including constitutive and regulated exocytosis. The role of exocytosis in a...

  • The molecular machinery of synaptic vesicle exocytosis. Li, L.; Chin, L.-S. // Cellular & Molecular Life Sciences;May2003, Vol. 60 Issue 5, p942 

    : At the synapse, neurotransmitters are released via Ca2+-triggered exocytotic fusion of synaptic vesicles with the presynaptic plasma membrane. Synaptic vesicle exocytosis seems to share many basic principles and homologous proteins with other membrane fusion events. Conserved components of the...

  • Caspase-independent cell death. Kroemer, Guido; Martin, Seamus J. // Nature Medicine;Jul2005, Vol. 11 Issue 7, p725 

    Caspase activation has been frequently viewed as synonymous with apoptotic cell death; however, caspases can also contribute to processes that do not culminate in cell demise. Moreover, inhibition of caspases can have cytoprotective effects. In a number of different models, caspase inhibition...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics