A simulation study on the Ca2+-independent but voltage-dependent exocytosis and endocytosis in dorsal root ganglion neurons

Hua Yang; Chen Zhang; Hui Zheng; Wei Xiong; Zhuan Zhou; Tao Xu; Jiu Ding
November 2005
European Biophysics Journal;Nov2005, Vol. 34 Issue 8, p1007
Academic Journal
In patch-clamped somata of dorsal root ganglion (DRG) neurons, two types of secretion have been proposed: Ca2+-dependent secretion and Ca2+-independent but voltage-dependent secretion (CIVDS). The Ca2+-induced and the depolarization-induced membrane capacitance ( C m) increases contribute 80 and 20% to the total C m increase, respectively (Zhang and Zhou in Nat Neurosci 5:425, ). In order to explore the mechanism of the voltage-dependent C m change (Δ C m), we constructed a model with sequential states. The simulation with this model closely approximates all the experimental data. The model predicts that the majority of fusion events (approximately 80%) are so-called “kiss-and-run” events, which account for the fast recovery or the rapid retrieval feature of the signals. The remaining 20% are attributed to full fusion events, which account for a slow retrieval feature. On the basis of the model, one mechanism of the activity-dependent endocytosis has revealed a differential distribution of vesicles between the kiss-and-run and full fusion states at different stimulation frequencies. The quantitative model presented in this study may help us to understand the mechanism of the CIVDS and the tightly coupled endocytosis found in mammalian DRG neurons.


Related Articles

  • Calcium dependence of action potential-induced endocytosis in chromaffin cells. Chan, Shyue-An; Chow, Robert; Smith, Corey // Pflugers Archiv European Journal of Physiology;Feb2003, Vol. 445 Issue 5, p540 

    Exocytosis occurs via fusion of transmitter-containing granules with the cell membrane, whereupon the granule contents are released and the cell membrane surface area increases. Exocytosis is followed by endocytosis to maintain proper cell membrane surface area and composition. We have shown...

  • High calcium concentrations shift the mode of exocytosis to the kiss-and-run mechanism. Alés, Eva; Tabares, Lucía; Poyato, Juan M.; Valero, Vicente; Lindau, Manfred; Alvarez de Toledo, Guillermo // Nature Cell Biology;May99, Vol. 1 Issue 1, p40 

    Exocytosis, the fusion of secretory vesicles with the plasma membrane to allow release of the contents of the vesicles into the extracellular environment, and endocytosis, the internalization of these vesicles to allow another round of secretion, are coupled. It is, however, uncertain whether...

  • Vesicular proteins exocytosed and subsequently retrieved by compensatory endocytosis are nonidentical. Wienisch, Martin; Klingauf, Jurgen // Nature Neuroscience;Aug2006, Vol. 9 Issue 8, p1019 

    Upon exocytosis, synaptic vesicle proteins are released into the plasma membrane and have to be retrieved by compensatory endocytosis. When green fluorescent protein–labeled versions of the vesicle proteins synaptobrevin-2 and synaptotagmin-1 are overexpressed in rat hippocampal neurons,...

  • Visinin-like proteins (VSNLs): interaction partners and emerging functions in signal transduction of a subfamily of neuronal Ca2+-sensor proteins. Braunewell, Karl-Heinz; Szanto, Andres // Cell & Tissue Research;Jan2009, Vol. 335 Issue 2, p301 

    The visinin-like protein (VSNL) subfamily, including VILIP-1 (the founder protein), VILIP-2, VILIP-3, hippocalcin, and neurocalcin δ, constitute a highly homologous subfamily of neuronal calcium sensor (NCS) proteins. Comparative studies have shown that VSNLs are expressed predominantly in...

  • The trypanosome flagellar pocket. Field, Mark C.; Carrington, Mark // Nature Reviews Microbiology;Nov2009, Vol. 7 Issue 11, p775 

    Trypanosomes are important disease agents and excellent models for the study of evolutionary cell biology. The trypanosome flagellar pocket is a small invagination of the plasma membrane where the flagellum exits the cytoplasm and participates in many cellular processes. It is the only site of...

  • Actin and dynamin recruitment and the lack thereof at exo- and endocytotic sites in PC12 cells. Felmy, Felix // Pflugers Archiv European Journal of Physiology;Jun2009, Vol. 458 Issue 2, p403 

    Protein recruitment during endocytosis is well characterized in fibroblasts. Since fibroblasts do not engage in regulated exocytosis, only information about protein recruitment during constitutive endocytosis is provided. Furthermore, the cortical actin of fibroblasts is characterized by stress...

  • Peeping at the vesicle kiss. Fesce, Riccardo; Meldolesi, Jacopo // Nature Cell Biology;May99, Vol. 1 Issue 1, pE3 

    Kiss-and-run fusion, a quick way of coupling exocytotic vesicle fusion to vesicle recycling by endocytosis, can allow full discharge of secretory-vesicle contents, such as neurotransmitters, and is more common than classical endocytosis at high calcium concentrations.

  • The molecular machinery of synaptic vesicle exocytosis. Li, L.; Chin, L.-S. // Cellular & Molecular Life Sciences;May2003, Vol. 60 Issue 5, p942 

    : At the synapse, neurotransmitters are released via Ca2+-triggered exocytotic fusion of synaptic vesicles with the presynaptic plasma membrane. Synaptic vesicle exocytosis seems to share many basic principles and homologous proteins with other membrane fusion events. Conserved components of the...

  • The COPII cage: unifying principles of vesicle coat assembly. Gürkan, Cemal; Stagg, Scott M.; LaPointe, Paul; Balch, William E. // Nature Reviews Molecular Cell Biology;Oct2006, Vol. 7 Issue 10, p727 

    Communication between compartments of the exocytic and endocytic pathways in eukaryotic cells involves transport carriers — vesicles and tubules — that mediate the vectorial movement of cargo. Recent studies of transport-carrier formation in the early secretory pathway have...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics