Rapid estimation of sugar release from winter wheat straw during bioethanol production using FTIR-photoacoustic spectroscopy

Bekiaris, Georgios; Lindedam, Jane; Peltre, Clément; Decker, Stephen R.; Turner, Geoffrey B.; Magid, Jakob; Bruun, Sander
June 2015
Biotechnology for Biofuels;2015, Vol. 8 Issue 1, p1
Academic Journal
Background: Complexity and high cost are the main limitations for high-throughput screening methods for the estimation of the sugar release from plant materials during bioethanol production. In addition, it is important that we improve our understanding of the mechanisms by which different chemical components are affecting the degradability of plant material. In this study, Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was combined with advanced chemometrics to develop calibration models predicting the amount of sugars released after pretreatment and enzymatic hydrolysis of wheat straw during bioethanol production, and the spectra were analysed to identify components associated with recalcitrance. Results: A total of 1122 wheat straw samples from nine different locations in Denmark and one location in the United Kingdom, spanning a large variation in genetic material and environmental conditions during growth, were analysed. The FTIR-PAS spectra of non-pretreated wheat straw were correlated with the measured sugar release, determined by a high-throughput pretreatment and enzymatic hydrolysis (HTPH) assay. A partial least square regression (PLSR) calibration model predicting the glucose and xylose release was developed. The interpretation of the regression coefficients revealed a positive correlation between the released glucose and xylose with easily hydrolysable compounds, such as amorphous cellulose and hemicellulose. Additionally, a negative correlation with crystalline cellulose and lignin, which inhibits cellulose and hemicellulose hydrolysis, was observed. Conclusions: FTIR-PAS was used as a reliable method for the rapid estimation of sugar release during bioethanol production. The spectra revealed that lignin inhibited the hydrolysis of polysaccharides into monomers, while the crystallinity of cellulose retarded its hydrolysis into glucose. Amorphous cellulose and xylans were found to contribute significantly to the released amounts of glucose and xylose, respectively.


Related Articles

  • Effects of Dilute Alkali Pretreatment on Chemical Components and Fermentable Sugars and Structure of Bamboo. Shangxing Chen; Zongde Wang; Kai Fang; Guorong Fan; Peng Wang // Advanced Materials Research;2014, Issue 1033-1034, p114 

    The pretreatment of bamboo with dilute alkali and the effects on chemical components and enzymatic hydrolysis were studied, and the fiber structural properties of pretreated residues were studied with Scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR). The...

  • Evaluation of Hydrolytic Activity of Different Pectinases on Sugar Beet (Beta vulgaris) Substrate Using FT-MIR Spectroscopy. Chiş, Adina; Fetea, Florinela; Matei, Horea; Socaciu, Carmen // Notulae Botanicae Horti Agrobotanici Cluj-Napoca;2011, Vol. 39 Issue 2, p99 

    The aim of this study was to evaluate the pectinase activity of two commercial enzymes Rohament PL and Rohapect 10L, using as substrate the sugar beet pulp, in different conditions. The method applied to check the rate of hydrolysis was Fourier-transformed infrared spectroscopy (FT-MIR), by...

  • Enhanced Biological Straw Saccharification Through Coculturing of Lignocellulose-Degrading Microorganisms. Taha, Mohamed; Shahsavari, Esmaeil; Al-Hothaly, Khalid; Mouradov, Aidyn; Smith, Andrew; Ball, Andrew; Adetutu, Eric // Applied Biochemistry & Biotechnology;Apr2015, Vol. 175 Issue 8, p3709 

    Lignocellulosic waste (LCW) is an abundant, low-cost, and inedible substrate for the induction of lignocellulolytic enzymes for cellulosic bioethanol production using an efficient, environmentally friendly, and economical biological approach. In this study, 30 different lignocellulose-degrading...

  • Broadly tunable quantum cascade laser in cantilever-enhanced photoacoustic infrared spectroscopy of solids. Lehtinen, J.; Kuusela, T. // Applied Physics B: Lasers & Optics;Jun2014, Vol. 115 Issue 3, p413 

    An external cavity quantum cascade laser (EC-QCL) is applied in the photoacoustic detection of solid samples. The EC-QCL used has a broad tuning range of 676 cm (970-1,646 cm) in the mid-infrared region, which enables accurate broadband spectroscopy of large molecules. The high spectral power...

  • Identification and Measurement of Intermolecular Interaction in Polyester/Polystyrene Blends by FTIR-Photoacoustic Spectrometry. Gordon, S.; Mohamed, A.; Harry-Okuru, R.; Biresaw, G. // Journal of Polymers & the Environment;Dec2015, Vol. 23 Issue 4, p459 

    Fourier transform infrared photoacoustic spectrometry was used to reveal and identify n-Ï€ type intermolecular interaction formed in plastic comprising binary blends of polystyrene and a biodegradable polymer, either polylactic acid, polycaprolactone or...

  • Optoacoustic measurements of the porosity of paper samples with foxings. Balakhnina, I. A.; Brandt, N. N.; Chikishev, A. Yu.; Pelivanov, I. M.; Rebrikova, N. L. // Applied Physics Letters;10/22/2012, Vol. 101 Issue 17, p174101 

    The goal of this work is to investigate the porosity of old paper with foxings. Initially, paper samples are characterized using the conventional absorption and Fourier transform infrared spectroscopies. Optoacoustic method based on laser excitation of short ultrasound transients is used for...

  • Thermo-oxidative Degradation Assessment in Quasi-isotropic Carbon Fiber/Epoxy Composites. Daily, Connor; Barnard, Dan J.; Jones, Roger W.; McClelland, John F.; Bowler, Nicola // AIP Conference Proceedings;2015, Vol. 1650 Issue 1, p1211 

    Components made from polymer matrix composites (PMCs) are finding increasing use in armored vehicles for the purpose of weight savings and fuel efficiency. Often times, these PMC components are installed next to engines, or in other high-temperature environments within the vehicle. The present...

  • Adsorption of congo red using ethylenediamine modified wheat straw. Zhanwu Wang; Pan Han; Yubin Jiao; Dan Ma; Chanchan Dou; Runping Han // Desalination & Water Treatment;Jun2011, Vol. 30 Issue 1-3, p195 

    The natural wheat straw (NWS) was modified by ethylenediamine and the modified wheat straw (MWS) was used as adsorbent for removal of congo red (CR) from aqueous solution. Analysis of FTIR and nitrogen element showed that amino group was introduced to NWS. A batch system was applied to study the...

  • Variation in the chemical composition of wheat straw: the role of tissue ratio and composition. Collins, Samuel; Wellner, Nikolaus; Bordonado, Isabel Martinez; Harper, Andrea; Miller, Charlotte; Bancroft, Ian; Waldron, Keith // Biotechnology for Biofuels;2014, Vol. 7 Issue 1, p1 

    Background Wheat straw is an attractive substrate for second generation ethanol production because it will complement and augment wheat production rather than competing with food production. However, like other sources of lignocellulosic biomass, even from a single species, it is heterogeneous...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics