TECHNOLOGY: Cre-ative mouse mosaics

Alfred, Jane
February 2002
Nature Reviews Genetics;Feb2002, Vol. 3 Issue 2, p88
Academic Journal
Discusses research being done on a Cre/loxP-based strategy that induced mitotic recombination in mouse embryonic stem cells. Reference to a study by Liu et al, published in the 2002 issue of "Nature Genetics"; Recombination frequencies.


Related Articles

  • Efficient Cre-loxP?induced mitotic recombination in mouse embryonic stem cells. Liu, Pentao; Jenkins, Nancy A.; Copeland, Neal G. // Nature Genetics;Jan2002, Vol. 30 Issue 1, p66 

    FLP/FRT-induced mitotic recombination provides a powerful method for creating genetic mosaics in Drosophila and for discerning the function of recessive genes in a heterozygous individual. Here we show that mitotic recombination can be reproducibly induced in mouse embryonic stem (ES) cells, by...

  • Homologous recombination in human embryonic stem cells. Zwaka, Thomas P.; Thomson, James A. // Nature Biotechnology;Mar2003, Vol. 21 Issue 3, p319 

    Homologous recombination applied to mouse embryonic stem (ES) cells has revolutionized the study of gene function in mammals. Although most often used to generate knockout mice, homologous recombination has also been applied in mouse ES cells allowed to differentiate in vitro. Homologous...

  • Efficient gene targeting in mouse embryonic stem cells. Templeton, N S; Roberts, D D; Safer, B // Gene Therapy;Jul97, Vol. 4 Issue 7, p700 

    We developed methods to improve the efficiency of gene correction in mouse embryonic stem cells using homologous recombination of a replacement vector. The absolute frequency of homologous recombination in mouse embryonic stem (ES) cells, defined as the frequency of homologous recombination per...

  • Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles. Testa, Giuseppe; Zhang, Youming; Vintersten, Kristina; Benes, Vladimir; Pijnappel, W.W.M. Pim; Chambers, Ian; Smith, Andrew J.H.; Smith, Austin G.; Stewart, A. Francis // Nature Biotechnology;Apr2003, Vol. 21 Issue 4, p443 

    The mouse is the leading vertebrate model because its genome can be altered by both random transgenesis and homologous recombination with targeting constructs. Both approaches have been hindered by the size and site limitations implicit in conventional Escherichia coli DNA-engineering methods....

  • Adaptation to culture of human embryonic stem cells and oncogenesis in vivo. Baker, Duncan E. C.; Harrison, Neil J.; Maltby, Edna; Smith, Kath; Moore, Harry D.; Shaw, Pamela J.; Heath, Paul R.; Holden, Hazel; Andrews, Peter W. // Nature Biotechnology;Feb2007, Vol. 25 Issue 2, p207 

    The application of human embryonic stem cells (HESCs) to provide differentiated cells for regenerative medicine will require the continuous maintenance of the undifferentiated stem cells for long periods in culture. However, chromosomal stability during extended passaging cannot be guaranteed,...

  • Role for the Mammalian Swi5-Sfr1 Complex in DNA Strand Break Repair through Homologous Recombination.  // PLoS Genetics;Oct2010, Vol. 6 Issue 10, p1 

    No abstract available.

  • Efficient biallelic mutagenesis with Cre/loxP-mediated inter-chromosomal recombination. Koike, Hiroko; Hone, Kyoji; Fukuyama, Hidehiro; Kondoh, Gen; Nagata, Shigekazu; Takeda, Junji // EMBO Reports;May2002, Vol. 3 Issue 5, p433 

    The isolation of mutant cells with phenotypes caused by random mutagenesis has been hampered in mammalian cells because there are two alleles per gene and the disruption of both alleles is extremely rare. We describe a method for the efficient biallelic mutagenesis in embryonic stem cells. IoxP...

  • Current issues in mouse genome engineering. Glaser, Stefan; Anastassiadis, Konstantinos; Stewart, A. Francis // Nature Genetics;Nov2005, Vol. 37 Issue 11, p1187 

    The mouse is the foremost vertebrate experimental model because its genome can be precisely and variously engineered. Now that the mouse genome has been sequenced and annotated, the task of mutating each gene is feasible, and an international cooperation is providing mutated embryonic stem cells...

  • Knockout, Genetic. Bussiere, Jeanine L.; Bolon, Brad; Vohr, Hans-Werner // Encyclopedic Reference of Immunotoxicology;2005, p368 

    The article presents information on genetic knockout. It refers to gene-targeted animals that have been modified to lack a specific gene. The techniques used to knockout genes include homologous recombination. In conventional knockout technology, embryonic stem cells are cultured and challenged...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics