IDO1, IDO2 , MICA and MICB mRNA Expression in Isolated Adipose Derived Stem Cells (ASCs) from Breast Cancer Patients Compared to Normal Breast Tissues

Razmkhah, M.; Ghaderi, A.; Bahrami, B.
June 2013
Cell Journal (Yakhteh);Summer 2013 Supplement 1, Vol. 15 Issue Sup 1, p74
Academic Journal
Objective: Adipose derived stem cells (ASCs) are detected in the tumor microenvironment and have immunomodulatory effects on both innate and adaptive immune responses. ASC-mediated immunesuppression is mainly induced through the secretion of soluble molecules such as IDO1, IDO2, MICA, MICB, NO, PGE2 and HLAG5. To evaluate the mRNA expression of IDO1, IDO2, MICA & MICB in adipose derived stem cells isolated from breast cancer patients and compare them to those of normal breast tissues. Materials and Methods: ASCs were isolated from 20 breast cancer patients and 10 normal breast adipose tissues obtained by cosmetic mamoplasty using collagenase digestion. The specified genes were assessed by q-RT- PCR method. Results: IDO1 gene expression in ASC cells from breast cancer patients was 2.3 fold more than that of ASCs from normal breasts. mRNA expression of the other genes including IDO2, MICA and MICB, was respectively 2.9 fold, 1.2 fold and 1.9 fold more than those of ASCs from normal breasts. However there was no significant difference between the expression of the indicated genes in ASCs of breast cancer patients and those of normal individuals. Conclusion: In the current study, the mRNA expression of IDO1, IDO2, MICA and MICB had no significant difference between breast cancer and normal breast tissue. This could be because of the relatively small number of samples in each group; therefore further studies on larger number of samples are needed to shed more light on this issue


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